Progress in the methodological strategies for the detection in real samples of desmosine and isodesmosine, two biological markers of elastin degradation

Simona Viglio; Laura Annovazzi; Maurizio Luisetti; Jan Stolk; Begoña Casado; Paolo Iadarola

doi:10.1002/jssc.200600260

Progress in the methodological strategies for the detection in real samples of desmosine and isodesmosine, two biological markers of elastin degradation

Simona Viglio, Laura Annovazzi, Maurizio Luisetti, Jan Stolk, Begoña Casado, Paolo Iadarola

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

Desmosines are crosslinking amino acids unique to mature elastin in humans. Owing to this unicity, they have been discussed as potentially attractive indicators of connective tissue disorders whose clinical manifestations are mostly the result of elastin degradation. This review covers advances in immunochemical, chromatographic, and electrophoretic procedures applied in the last 25 years to detect and quantitate these crosslinks in a variety of biological samples. Recent applications of CE with LIF detection (CE-LIF) for investigating the content of desmosines in different fluids will also be discussed.

Original language	English
Pages (from-to)	202-213
Number of pages	12
Journal	Journal of Separation Science
Volume	30
Issue number	2
DOIs	https://doi.org/10.1002/jssc.200600260
Publication status	Published - Feb 2007

Keywords

Biological fluids
Biomarkers
Desmosine
Isodesmosine

ASJC Scopus subject areas

Clinical Biochemistry
Molecular Medicine
Chemical Engineering(all)
Filtration and Separation

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10.1002/jssc.200600260

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Progress in the methodological strategies for the detection in real samples of desmosine and isodesmosine, two biological markers of elastin degradation. / Viglio, Simona; Annovazzi, Laura; Luisetti, Maurizio; Stolk, Jan; Casado, Begoña; Iadarola, Paolo.

In: Journal of Separation Science, Vol. 30, No. 2, 02.2007, p. 202-213.

Research output: Contribution to journal › Article › peer-review

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