Processualità del quadro ematico periferico nella anemia emolitica da fenilidrazina.

Translated title of the contribution: Progression of the peripheral blood profile in phenylhydrazine-induced hemolytic anemia

G. Piacentini, L. Baronciani, S. Rapa, C. Benedetti, P. Ninfali

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Male rabbits were made anemic by subcutaneous injections of 3 mg/Kg body weight of phenylhydrazine (PHZ) for the duration of three days. Blood samples were collected prior to the experiment, on the 2nd and 5th day from the end of the treatment. Hematocrit, reticulocyte count, MCV, osmotic resistance, RBC volume distribution curve and morphological analysis were performed on each sample in order to obtain a picture of the progressive changes of the parameters following the PHZ administration. Hematocrit values changed from 49 to 27%, whereas the reticulocytosis increased from 0.6 to 73%. A significant elevation of MCV was detected and the cell volume distribution curve, which presented the typical bell-shape profile in the normal animals became bimodal on the 5th day. The osmotic resistance of RBC of anemic animals, showed a marked deviation from the normal pattern. In fact initial haemolysis started at 0.75-0.70% NaCl concentration in the 5th day samples, while it was between 0.55-0.50% before the PHZ treatment. Finally, morphological analysis revealed a progressive increase of RBC with Heinz bodies, of macro-megalocytes and of immature erythroblasts thus indicating that the cell population, produced during recovery from PHZ induced anemia, is widely heterogeneous.

Original languageItalian
Pages (from-to)729-734
Number of pages6
JournalBollettino della Societa Italiana di Biologia Sperimentale
Volume66
Issue number8
Publication statusPublished - Aug 1990

Fingerprint

Hemolytic Anemia
Hematocrit
Heinz Bodies
Reticulocytosis
Reticulocyte Count
Erythroblasts
Subcutaneous Injections
Hemolysis
Cell Size
Anemia
Body Weight
Rabbits
phenylhydrazine
Therapeutics
Population

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Processualità del quadro ematico periferico nella anemia emolitica da fenilidrazina. / Piacentini, G.; Baronciani, L.; Rapa, S.; Benedetti, C.; Ninfali, P.

In: Bollettino della Societa Italiana di Biologia Sperimentale, Vol. 66, No. 8, 08.1990, p. 729-734.

Research output: Contribution to journalArticle

@article{3bb09e7114dd4e3785beb223859bb50c,
title = "Processualit{\`a} del quadro ematico periferico nella anemia emolitica da fenilidrazina.",
abstract = "Male rabbits were made anemic by subcutaneous injections of 3 mg/Kg body weight of phenylhydrazine (PHZ) for the duration of three days. Blood samples were collected prior to the experiment, on the 2nd and 5th day from the end of the treatment. Hematocrit, reticulocyte count, MCV, osmotic resistance, RBC volume distribution curve and morphological analysis were performed on each sample in order to obtain a picture of the progressive changes of the parameters following the PHZ administration. Hematocrit values changed from 49 to 27{\%}, whereas the reticulocytosis increased from 0.6 to 73{\%}. A significant elevation of MCV was detected and the cell volume distribution curve, which presented the typical bell-shape profile in the normal animals became bimodal on the 5th day. The osmotic resistance of RBC of anemic animals, showed a marked deviation from the normal pattern. In fact initial haemolysis started at 0.75-0.70{\%} NaCl concentration in the 5th day samples, while it was between 0.55-0.50{\%} before the PHZ treatment. Finally, morphological analysis revealed a progressive increase of RBC with Heinz bodies, of macro-megalocytes and of immature erythroblasts thus indicating that the cell population, produced during recovery from PHZ induced anemia, is widely heterogeneous.",
author = "G. Piacentini and L. Baronciani and S. Rapa and C. Benedetti and P. Ninfali",
year = "1990",
month = "8",
language = "Italian",
volume = "66",
pages = "729--734",
journal = "Bollettino della Societa Italiana di Biologia Sperimentale",
issn = "0037-8771",
publisher = "Casa Editrice Idelson Gnocchi",
number = "8",

}

TY - JOUR

T1 - Processualità del quadro ematico periferico nella anemia emolitica da fenilidrazina.

AU - Piacentini, G.

AU - Baronciani, L.

AU - Rapa, S.

AU - Benedetti, C.

AU - Ninfali, P.

PY - 1990/8

Y1 - 1990/8

N2 - Male rabbits were made anemic by subcutaneous injections of 3 mg/Kg body weight of phenylhydrazine (PHZ) for the duration of three days. Blood samples were collected prior to the experiment, on the 2nd and 5th day from the end of the treatment. Hematocrit, reticulocyte count, MCV, osmotic resistance, RBC volume distribution curve and morphological analysis were performed on each sample in order to obtain a picture of the progressive changes of the parameters following the PHZ administration. Hematocrit values changed from 49 to 27%, whereas the reticulocytosis increased from 0.6 to 73%. A significant elevation of MCV was detected and the cell volume distribution curve, which presented the typical bell-shape profile in the normal animals became bimodal on the 5th day. The osmotic resistance of RBC of anemic animals, showed a marked deviation from the normal pattern. In fact initial haemolysis started at 0.75-0.70% NaCl concentration in the 5th day samples, while it was between 0.55-0.50% before the PHZ treatment. Finally, morphological analysis revealed a progressive increase of RBC with Heinz bodies, of macro-megalocytes and of immature erythroblasts thus indicating that the cell population, produced during recovery from PHZ induced anemia, is widely heterogeneous.

AB - Male rabbits were made anemic by subcutaneous injections of 3 mg/Kg body weight of phenylhydrazine (PHZ) for the duration of three days. Blood samples were collected prior to the experiment, on the 2nd and 5th day from the end of the treatment. Hematocrit, reticulocyte count, MCV, osmotic resistance, RBC volume distribution curve and morphological analysis were performed on each sample in order to obtain a picture of the progressive changes of the parameters following the PHZ administration. Hematocrit values changed from 49 to 27%, whereas the reticulocytosis increased from 0.6 to 73%. A significant elevation of MCV was detected and the cell volume distribution curve, which presented the typical bell-shape profile in the normal animals became bimodal on the 5th day. The osmotic resistance of RBC of anemic animals, showed a marked deviation from the normal pattern. In fact initial haemolysis started at 0.75-0.70% NaCl concentration in the 5th day samples, while it was between 0.55-0.50% before the PHZ treatment. Finally, morphological analysis revealed a progressive increase of RBC with Heinz bodies, of macro-megalocytes and of immature erythroblasts thus indicating that the cell population, produced during recovery from PHZ induced anemia, is widely heterogeneous.

UR - http://www.scopus.com/inward/record.url?scp=0025467991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025467991&partnerID=8YFLogxK

M3 - Articolo

C2 - 2291827

AN - SCOPUS:0025467991

VL - 66

SP - 729

EP - 734

JO - Bollettino della Societa Italiana di Biologia Sperimentale

JF - Bollettino della Societa Italiana di Biologia Sperimentale

SN - 0037-8771

IS - 8

ER -