Abstract
We have previously shown that infection of CD4+ T lymphocytes with the T-lymphotropic human herpesvirus 7 (HHV-7) downregulates surface CD4, which represents the high-affinity receptor for HHV-7. In this study, we report that HHV-7 infection also causes a progressive loss of the surface CXC- chemokine receptor 4 (CXCR4) in CD4+ T cells, accompanied by a reduced intracellular Ca2+ flux and chemotaxis in response to stromal cell-derived factor-1α (SDF-1α), the specific CXCR4 ligand. Moreover, CXCR4 is downregulated from the surface of HHV-7-infected T cells independently of CD4. Because intracellular CXCR4 antigen and mRNA levels are unaffected in productively HHV-7-infected cells, the downregulation of CXCR4 apparently does not involve a transcritional block. Since CXCR4 functions in association with CD4 to permit entry of several human immunodeficiency virus (HIV) isolates, the potential of HHV-7 to persistently downregulate the surface expression of CXCR4 may provide novel strategies for limiting HIV infection.
| Original language | English |
|---|---|
| Pages (from-to) | 4521-4528 |
| Number of pages | 8 |
| Journal | Blood |
| Volume | 92 |
| Issue number | 12 |
| Publication status | Published - Dec 15 1998 |
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ASJC Scopus subject areas
- Hematology
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Progressive and persistent downregulation of surface CXCR4 in CD4+ T cells infected with human herpesvirus 7. / Secchiero, Paola; Zella, Davide; Barabitskaja, Oxana; Reitz, Marvin S.; Capitani, Silvano; Gallo, Robert C.; Zauli, Giorgio.
In: Blood, Vol. 92, No. 12, 15.12.1998, p. 4521-4528.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Progressive and persistent downregulation of surface CXCR4 in CD4+ T cells infected with human herpesvirus 7
AU - Secchiero, Paola
AU - Zella, Davide
AU - Barabitskaja, Oxana
AU - Reitz, Marvin S.
AU - Capitani, Silvano
AU - Gallo, Robert C.
AU - Zauli, Giorgio
PY - 1998/12/15
Y1 - 1998/12/15
N2 - We have previously shown that infection of CD4+ T lymphocytes with the T-lymphotropic human herpesvirus 7 (HHV-7) downregulates surface CD4, which represents the high-affinity receptor for HHV-7. In this study, we report that HHV-7 infection also causes a progressive loss of the surface CXC- chemokine receptor 4 (CXCR4) in CD4+ T cells, accompanied by a reduced intracellular Ca2+ flux and chemotaxis in response to stromal cell-derived factor-1α (SDF-1α), the specific CXCR4 ligand. Moreover, CXCR4 is downregulated from the surface of HHV-7-infected T cells independently of CD4. Because intracellular CXCR4 antigen and mRNA levels are unaffected in productively HHV-7-infected cells, the downregulation of CXCR4 apparently does not involve a transcritional block. Since CXCR4 functions in association with CD4 to permit entry of several human immunodeficiency virus (HIV) isolates, the potential of HHV-7 to persistently downregulate the surface expression of CXCR4 may provide novel strategies for limiting HIV infection.
AB - We have previously shown that infection of CD4+ T lymphocytes with the T-lymphotropic human herpesvirus 7 (HHV-7) downregulates surface CD4, which represents the high-affinity receptor for HHV-7. In this study, we report that HHV-7 infection also causes a progressive loss of the surface CXC- chemokine receptor 4 (CXCR4) in CD4+ T cells, accompanied by a reduced intracellular Ca2+ flux and chemotaxis in response to stromal cell-derived factor-1α (SDF-1α), the specific CXCR4 ligand. Moreover, CXCR4 is downregulated from the surface of HHV-7-infected T cells independently of CD4. Because intracellular CXCR4 antigen and mRNA levels are unaffected in productively HHV-7-infected cells, the downregulation of CXCR4 apparently does not involve a transcritional block. Since CXCR4 functions in association with CD4 to permit entry of several human immunodeficiency virus (HIV) isolates, the potential of HHV-7 to persistently downregulate the surface expression of CXCR4 may provide novel strategies for limiting HIV infection.
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M3 - Article
VL - 92
SP - 4521
EP - 4528
JO - Blood
JF - Blood
SN - 0006-4971
IS - 12
ER -