TY - JOUR
T1 - Proinflammatory cytokines, adhesion molecules, and lymphocyte integrin expression in the internal jugular blood of migraine patients without aura assessed ictally
AU - Sarchielli, Paola
AU - Alberti, Andrea
AU - Baldi, Antonio
AU - Coppola, Francesca
AU - Rossi, Cristiana
AU - Pierguidi, Laura
AU - Floridi, Alessandro
AU - Calabresi, Paolo
PY - 2006/2
Y1 - 2006/2
N2 - Objective. - The aim of the present research was to verify the levels of the soluble adhesion molecules sL- and sE-selectins, intercellular adhesion molecule (sICAM)-1, and vascular cell adhesion molecule-1 in serial samples of internal jugular venous blood taken from migraine patients without aura (MWoA) during attacks. The expression of leukocyte function antigen (LFA)-1 and very late activation antigen (VLA)-4 was also assessed on lymphocytes obtained from jugular venous blood. Levels of certain proinflammatory cytokines (tumor necrosis factor-α[TNF-α], interleukin-1β[IL-1β], IL-4, and IL-6) were also determined and correlated with those of adhesion molecules. Patients and Methods. - Seven MWoA patients were admitted in the hospital during attacks and blood samples were taken immediately after catheter insertion, at 1, 2, and 4 hours after attack onset, and within 2 hours after its termination. The levels of adhesion molecules and cytokines were measured with ELISA method. The expression of LFA-1 and VLA-4 was assessed by flow cytometry. Results. - A parallel transient increase of sICAM-1, TNF-α, and IL-6 was observed in the first 2 hours after attack onset compared with the time of catheter insertion (P <.0001,
AB - Objective. - The aim of the present research was to verify the levels of the soluble adhesion molecules sL- and sE-selectins, intercellular adhesion molecule (sICAM)-1, and vascular cell adhesion molecule-1 in serial samples of internal jugular venous blood taken from migraine patients without aura (MWoA) during attacks. The expression of leukocyte function antigen (LFA)-1 and very late activation antigen (VLA)-4 was also assessed on lymphocytes obtained from jugular venous blood. Levels of certain proinflammatory cytokines (tumor necrosis factor-α[TNF-α], interleukin-1β[IL-1β], IL-4, and IL-6) were also determined and correlated with those of adhesion molecules. Patients and Methods. - Seven MWoA patients were admitted in the hospital during attacks and blood samples were taken immediately after catheter insertion, at 1, 2, and 4 hours after attack onset, and within 2 hours after its termination. The levels of adhesion molecules and cytokines were measured with ELISA method. The expression of LFA-1 and VLA-4 was assessed by flow cytometry. Results. - A parallel transient increase of sICAM-1, TNF-α, and IL-6 was observed in the first 2 hours after attack onset compared with the time of catheter insertion (P <.0001,
KW - Intercellular adhesion molecule-1
KW - Interleukin-1β
KW - Interleukin-6
KW - Internal jugular blood
KW - Migraine without aura
KW - Tumor necrosis factor-α
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U2 - 10.1111/j.1526-4610.2006.00337.x
DO - 10.1111/j.1526-4610.2006.00337.x
M3 - Article
C2 - 16492228
AN - SCOPUS:33645070198
VL - 46
SP - 200
EP - 207
JO - Headache
JF - Headache
SN - 0017-8748
IS - 2
ER -