Proinflammatory cytokines, adhesion molecules, and lymphocyte integrin expression in the internal jugular blood of migraine patients without aura assessed ictally

Paola Sarchielli, Andrea Alberti, Antonio Baldi, Francesca Coppola, Cristiana Rossi, Laura Pierguidi, Alessandro Floridi, Paolo Calabresi

Research output: Contribution to journalArticlepeer-review

Abstract

Objective. - The aim of the present research was to verify the levels of the soluble adhesion molecules sL- and sE-selectins, intercellular adhesion molecule (sICAM)-1, and vascular cell adhesion molecule-1 in serial samples of internal jugular venous blood taken from migraine patients without aura (MWoA) during attacks. The expression of leukocyte function antigen (LFA)-1 and very late activation antigen (VLA)-4 was also assessed on lymphocytes obtained from jugular venous blood. Levels of certain proinflammatory cytokines (tumor necrosis factor-α[TNF-α], interleukin-1β[IL-1β], IL-4, and IL-6) were also determined and correlated with those of adhesion molecules. Patients and Methods. - Seven MWoA patients were admitted in the hospital during attacks and blood samples were taken immediately after catheter insertion, at 1, 2, and 4 hours after attack onset, and within 2 hours after its termination. The levels of adhesion molecules and cytokines were measured with ELISA method. The expression of LFA-1 and VLA-4 was assessed by flow cytometry. Results. - A parallel transient increase of sICAM-1, TNF-α, and IL-6 was observed in the first 2 hours after attack onset compared with the time of catheter insertion (P <.0001,

Original languageEnglish
Pages (from-to)200-207
Number of pages8
JournalHeadache
Volume46
Issue number2
DOIs
Publication statusPublished - Feb 2006

Keywords

  • Intercellular adhesion molecule-1
  • Interleukin-1β
  • Interleukin-6
  • Internal jugular blood
  • Migraine without aura
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

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