Proinflammatory profile of cytokine production by human monocytes and murine microglia stimulated with β-amyloid[25-35]

Lucia Meda, Pierluigi Baron, Elisabetta Prat, Elio Scarpini, Guglielmo Scarlato, Marco Antonio Cassatella, Filippo Rossi

Research output: Contribution to journalArticlepeer-review

Abstract

Growing evidence indicates that amyloid (Aβ) deposition and phagocyte activation participate in inflammatory reactions in the brain during the course of Alzheimer's disease. To further investigate the effects of Aβ- phagocyte interaction, we examined the production of proinflammatory (IL- lβ, IL-6), chemotactic (MIP-1α, IP-10) and inhibitory (IL-1Ra, IL-10 and TGFβ1) cytokines by cultured human monocytes and mouse microglial cells upon stimulation with Aβ[25-35]. Northern blot analysis and specific immunoassays demonstrated that Aβ[125-35] triggers mRNA expression and release of IL-1β, IL-1Ra and MIP-1αbut not of IL-6, IL-10, TGFβ1 and IP- 10 from human monocytes. Similar results were obtained by examining the production of IL-1β, IL-6 and IL-10 from mouse microglial cells in the same experimental conditions. Taken together, these data indicate that Aβ- phagocyte interaction can drive a different response towards cytokine production by monocytes and microglia, with a particular proinflammatory trend, and further support a role for Aβ deposition as a triggering factor of inflammatory events in Alzheimer's disease.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalJournal of Neuroimmunology
Volume93
Issue number1-2
DOIs
Publication statusPublished - Jan 1 1999

Keywords

  • β-amyloid[25- 35]
  • Alzheimer's disease
  • Cytokines
  • Inflammation
  • Microglia
  • Monocytes

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

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