Proinflammatory role of glucocorticoid-induced TNF receptor-related gene in acute lung inflammation

Salvatore Cuzzocrea, Giuseppe Nocentini, Rosanna Di Paola, Massimiliano Agostini, Emanuela Mazzon, Simona Ronchetti, Concetta Crisafulli, Emanuela Esposito, Achille P. Caputi, Carlo Riccardi

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Abstract

Glucocorticoid-induced TNFR-related gene (GITR) participates in the immune/inflammatory response. Because GITR expression has been described in cells other than T lymphocytes, we investigated whether it also modulates acute inflammatory response. Using GITR-deficient (GITR-/-) mice, we analyzed the role of GITR in the development of carrageenan-induced lung inflammation (pleurisy) by studying several proinflammatory markers 2-8 h after carrageenan injection. When compared with GITR+/+, GITR-/- mice exhibited decreased production of turbid exudate containing a lower number of leukocytes. This was correlated with the reduction of inflammatory markers (including TNF-α, IL-1β, myeloperoxidase, inducible NO synthase, and cyclooxygenase 2) in the pleural exudate and/or in the lung. Moreover, endothelial cells expressed lower levels of adhesion molecules. In lungs of GITR+/+ mice, GITR ligand expression was not modulated during pleurisy, while that of GITR increased, as a consequence of increased infiltration by GITR-expressing cells and of GITR up-regulation in macrophages and endothelial cells. Finally, cotreatment of GITR+/+ mice with carrageenan and Fc-GITR fusion protein decreased the number of inflammatory cells (pleural macrophages and lung neutrophils) as compared with carrageenan treatment alone, confirming that GITR plays a role in the modulation of pleurisy.

Original languageEnglish
Pages (from-to)631-641
Number of pages11
JournalJournal of Immunology
Volume177
Issue number1
Publication statusPublished - Jul 1 2006

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Tumor Necrosis Factor Receptors
Glucocorticoids
Pneumonia
Genes
Carrageenan
Pleurisy
Exudates and Transudates
Lung
Endothelial Cells
Macrophages
Gene Expression
Gene Fusion
Cyclooxygenase 2
Interleukin-1
Leukocyte Count
Nitric Oxide Synthase
Peroxidase
Neutrophils

ASJC Scopus subject areas

  • Immunology

Cite this

Cuzzocrea, S., Nocentini, G., Di Paola, R., Agostini, M., Mazzon, E., Ronchetti, S., ... Riccardi, C. (2006). Proinflammatory role of glucocorticoid-induced TNF receptor-related gene in acute lung inflammation. Journal of Immunology, 177(1), 631-641.

Proinflammatory role of glucocorticoid-induced TNF receptor-related gene in acute lung inflammation. / Cuzzocrea, Salvatore; Nocentini, Giuseppe; Di Paola, Rosanna; Agostini, Massimiliano; Mazzon, Emanuela; Ronchetti, Simona; Crisafulli, Concetta; Esposito, Emanuela; Caputi, Achille P.; Riccardi, Carlo.

In: Journal of Immunology, Vol. 177, No. 1, 01.07.2006, p. 631-641.

Research output: Contribution to journalArticle

Cuzzocrea, S, Nocentini, G, Di Paola, R, Agostini, M, Mazzon, E, Ronchetti, S, Crisafulli, C, Esposito, E, Caputi, AP & Riccardi, C 2006, 'Proinflammatory role of glucocorticoid-induced TNF receptor-related gene in acute lung inflammation', Journal of Immunology, vol. 177, no. 1, pp. 631-641.
Cuzzocrea S, Nocentini G, Di Paola R, Agostini M, Mazzon E, Ronchetti S et al. Proinflammatory role of glucocorticoid-induced TNF receptor-related gene in acute lung inflammation. Journal of Immunology. 2006 Jul 1;177(1):631-641.
Cuzzocrea, Salvatore ; Nocentini, Giuseppe ; Di Paola, Rosanna ; Agostini, Massimiliano ; Mazzon, Emanuela ; Ronchetti, Simona ; Crisafulli, Concetta ; Esposito, Emanuela ; Caputi, Achille P. ; Riccardi, Carlo. / Proinflammatory role of glucocorticoid-induced TNF receptor-related gene in acute lung inflammation. In: Journal of Immunology. 2006 ; Vol. 177, No. 1. pp. 631-641.
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