Proliferation and adhesion capability of human gingival fibroblasts onto Zirconia, lithium disilicate and feldspathic veneering ceramic in vitro

Stefano Tetè, Vincenzo Luca Zizzari, Bruna Borelli, Marianna De Colli, Susi Zara, Roberto Sorrentino, Antonio Scarano, Enrico Gherlone, Amelia Cataldi, Fernando Zarone

Research output: Contribution to journalArticle


Human gingival fbroblasts (HGFs) were cultured onto CAD/CAM zirconia (Group A), CAD/CAM zirconia after polishing (Group B), CAD/CAM lithium disilicate after polishing (Group C), and feldspathic ceramic (Group D) to evaluate their proliferation and adhesion potential. After 3 h, HGF adhesion was similar in all groups. Later, HGFs closely adhered to surfaces, particularly onto groups B, C and D, acquiring an elongated shape. Proliferation assay showed no differences in cell viability among the groups after 24 h, while significant increase was shown after 72 h in Groups B and C. After 24 h, similar Collagen I levels were found in all groups, while after 72 h Groups B and C revealed a deep reduction in respect to the 24 h level. In vitro, HGF behavior may reflect variability in soft tissue response to different surface materials for prosthetic restorations, and support that polished zirconia is able to achieve a better integration in vivo in respect to the other materials.

Original languageEnglish
Pages (from-to)7-15
Number of pages9
JournalDental Materials Journal
Issue number1
Publication statusPublished - 2014



  • Feldspathic ceramic
  • Human gingival fbroblasts
  • Lithium disilicate
  • Type I collagen
  • Zirconia

ASJC Scopus subject areas

  • Dentistry(all)
  • Ceramics and Composites
  • Medicine(all)

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