Proliferation and cell loss of human leukemic cell subpopulations in liquid culture

P. A. Bernabei; F. C. Agostino; R. Bezzini; R. Saccardi; V. Gattei; V. Santini; M. Casini; P. Rossi Ferrini

doi:10.1007/BF01941723

Proliferation and cell loss of human leukemic cell subpopulations in liquid culture

P. A. Bernabei, F. C. Agostino, R. Bezzini, R. Saccardi, V. Gattei, V. Santini, M. Casini, P. Rossi Ferrini

Centro di Riferimento Oncologico

Research output: Contribution to journal › Article › peer-review

Abstract

A kinetic study was performed on leukemic blasts from patients with acute myeloid leukemia, separated into 2 subpopulations by a specific density gradient. The growth curve and the [³H]-thymidine uptake were simultaneously analyzed. While cumulative nucleotide uptake fitted with the growth kinetics in the low-density fraction, such a concordance was not found in the high-density subpopulation. That indicated the occurrence of simultaneous growth and loss in the high density fraction, which could not be evaluated by a simple numerical determination.

Original language	English
Pages (from-to)	245-247
Number of pages	3
Journal	Experientia
Volume	44
Issue number	3
DOIs	https://doi.org/10.1007/BF01941723
Publication status	Published - Mar 1988

Keywords

Acute myelogenous leukemia
cell loss
cell subpopulations
kinetics in culture

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1007/BF01941723

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abstract = "A kinetic study was performed on leukemic blasts from patients with acute myeloid leukemia, separated into 2 subpopulations by a specific density gradient. The growth curve and the [3H]-thymidine uptake were simultaneously analyzed. While cumulative nucleotide uptake fitted with the growth kinetics in the low-density fraction, such a concordance was not found in the high-density subpopulation. That indicated the occurrence of simultaneous growth and loss in the high density fraction, which could not be evaluated by a simple numerical determination.",

keywords = "Acute myelogenous leukemia, cell loss, cell subpopulations, kinetics in culture",

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AU - Bernabei, P. A.

AU - Agostino, F. C.

AU - Bezzini, R.

AU - Saccardi, R.

AU - Gattei, V.

AU - Santini, V.

AU - Casini, M.

AU - Rossi Ferrini, P.

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KW - Acute myelogenous leukemia

KW - cell loss

KW - cell subpopulations

KW - kinetics in culture

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Proliferation and cell loss of human leukemic cell subpopulations in liquid culture

Abstract

Keywords

ASJC Scopus subject areas

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