Proliferation of mouse primordial germ cells in Vitro

A key role for cAMP

Massimo De Felici, Susanna Dolci, Maurizio Pesce

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Two agents known to enhance the level of intracellular cAMP (dibutyryl cAMP and forskolin) markedly increase the number of 8.5, 10.5, and 11.5 days postcoitum (dpc) mouse primordial germ cells (PGCs) cultured on TM4 cell feeder layers. Forskolin (FRSK) caused a significant increase of PGC number also in monodispersed cell suspensions obtained from PGC-containing tissues of the three embryonic ages studied and in purified 11.5 dpc PGCs cultured without feeder layers. The addition to the culture medium of adenosine-3′,5′-cyclic monophosphorothioate RP isomer (Rp-cAMPS, a competitive antagonist for cAMP-dependent protein kinases), significantly reduced the effects of FRSK. Last, FRSK stimulated PGC proliferation, as assessed by 5-bromo-2′-deoxyuridin incorporation. We conclude that cAMP-dependent mechanisms play a crucial role in the control of mitotic proliferation of mouse PGCs in Culture.

Original languageEnglish
Pages (from-to)277-280
Number of pages4
JournalDevelopmental Biology
Volume157
Issue number1
Publication statusPublished - 1993

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Germ Cells
Colforsin
Feeder Cells
Cyclic AMP-Dependent Protein Kinases
Adenosine
Culture Media
In Vitro Techniques
Suspensions
Cell Culture Techniques
Cell Count
Cell Proliferation

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Proliferation of mouse primordial germ cells in Vitro : A key role for cAMP. / De Felici, Massimo; Dolci, Susanna; Pesce, Maurizio.

In: Developmental Biology, Vol. 157, No. 1, 1993, p. 277-280.

Research output: Contribution to journalArticle

De Felici, Massimo ; Dolci, Susanna ; Pesce, Maurizio. / Proliferation of mouse primordial germ cells in Vitro : A key role for cAMP. In: Developmental Biology. 1993 ; Vol. 157, No. 1. pp. 277-280.
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