Proliferative and/or cytotoxic activity of lymphocyte clones to autologous human melanoma

G. Fossati, A. Anichini, P. Squarcina, A. Mazzocchi, G. Parmiani

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15 Citations (Scopus)

Abstract

Peripheral blood lymphocytes (PBL) of a patient with metastatic melanoma were cultured with autologous melanoma cells (Auto-Me) and recombinant interleukin 2 (IL-2) (MLTC-PBL). Thirty-five days later, when no cytotoxicity against Auto-Me or K562 was detectable, MLTC-PBL were cloned in the presence of Auto-Me, IL-2 (25 U/ml) and Daudi cells as feeder. Eighty-one growing clones were simultaneously screened for proliferative and cytotoxic activity to Auto-Me. Twenty-two clones proliferated in the presence of Auto-Me only, 29 in the presence of IL-2 only and 41 in the presence of Auto-Me plus IL-2; 12 clones showed cytotoxic activity against Auto-Me. Six clones expressed both cytotoxic and proliferative activity to Auto-Me. The phenotype of 6 proliferative clones tested was CD3+, CD4+, WT31+, CD8-, CD16-, Leu19-, whereas that of 2 cytotoxic-proliferative clones tested was CD3+, CD8+, Leu19+, WT31+, CD4-, CD16-. Specificity analysis of proliferative response of 6 clones and of cytotoxicity of 7 clones, tested on a panel of 14 different target cells, revealed a complex pattern of reactivity, each clone expressing a peculiar specificity. Our results suggest the possibility of isolating, from melanoma patients' PBL, T-cell clones with proliferative activity to Auto-Me and Auto-Me plus IL-2, and T-cell clones which apparently express both proliferative and cytotoxic activity to Auto-Me.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalInternational Journal of Cancer
Volume42
Issue number2
Publication statusPublished - 1988

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Melanoma
Clone Cells
Lymphocytes
Interleukin-2
T-Lymphocytes
Feeder Cells
K562 Cells
Interleukin-17
Interleukin-12
Phenotype

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Proliferative and/or cytotoxic activity of lymphocyte clones to autologous human melanoma. / Fossati, G.; Anichini, A.; Squarcina, P.; Mazzocchi, A.; Parmiani, G.

In: International Journal of Cancer, Vol. 42, No. 2, 1988, p. 239-245.

Research output: Contribution to journalArticle

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T1 - Proliferative and/or cytotoxic activity of lymphocyte clones to autologous human melanoma

AU - Fossati, G.

AU - Anichini, A.

AU - Squarcina, P.

AU - Mazzocchi, A.

AU - Parmiani, G.

PY - 1988

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N2 - Peripheral blood lymphocytes (PBL) of a patient with metastatic melanoma were cultured with autologous melanoma cells (Auto-Me) and recombinant interleukin 2 (IL-2) (MLTC-PBL). Thirty-five days later, when no cytotoxicity against Auto-Me or K562 was detectable, MLTC-PBL were cloned in the presence of Auto-Me, IL-2 (25 U/ml) and Daudi cells as feeder. Eighty-one growing clones were simultaneously screened for proliferative and cytotoxic activity to Auto-Me. Twenty-two clones proliferated in the presence of Auto-Me only, 29 in the presence of IL-2 only and 41 in the presence of Auto-Me plus IL-2; 12 clones showed cytotoxic activity against Auto-Me. Six clones expressed both cytotoxic and proliferative activity to Auto-Me. The phenotype of 6 proliferative clones tested was CD3+, CD4+, WT31+, CD8-, CD16-, Leu19-, whereas that of 2 cytotoxic-proliferative clones tested was CD3+, CD8+, Leu19+, WT31+, CD4-, CD16-. Specificity analysis of proliferative response of 6 clones and of cytotoxicity of 7 clones, tested on a panel of 14 different target cells, revealed a complex pattern of reactivity, each clone expressing a peculiar specificity. Our results suggest the possibility of isolating, from melanoma patients' PBL, T-cell clones with proliferative activity to Auto-Me and Auto-Me plus IL-2, and T-cell clones which apparently express both proliferative and cytotoxic activity to Auto-Me.

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