Proline-rich tyrosine kinase 2 regulates proliferation and differentiation of prostate cells

Antonietta Picascia, Rosita Stanzione, Paolo Chieffi, Annamaria Kisslinger, Ivan Dikic, Donatella Tramontano

Research output: Contribution to journalArticle

Abstract

Proline-rich tyrosine kinase 2 (Pyk2) expression in prostate epithelium inversely correlated with degree of malignancy of prostate cancers, thus the role of Pyk2 in the regulation of prostate cells proliferation and differentiation was investigate in PC3 cells. Pyk2 can be activated by canonic stimuli such as tumor necrosis factorα and lysophosphatidic acid (LPA) in PC3 cells, in addition, LPA stimulated Pyk2 phosphorylation also induced extracellular signal-regulated kinase 1 and 2 activation in these cells. Proliferation of PC3 cell clones (PC3-PKM) expressing a dominant negative kinase-defective Pyk2 mutant is consistently decreased in respect to that of wild type PC3 cells. In addition, PC3-PKM clones underwent total block cell proliferation upon treatment with dibutyryl cAMP. Finally, in the presence of sustained levels of intracellular cAMP, PC3-PKM cells, but not wild type PC3 cells, acquired a neuron-like morphology. Taken together our results suggest that Pyk2 plays a role in the regulation of prostate cell proliferation and, more interestingly, its expression may represents a sensitive marker of prostate state of differentiation.

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume186
Issue number1
Publication statusPublished - Jan 15 2002

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Keywords

  • Neuroendocrine differentiation
  • Proline-rich tyrosine kinase 2
  • Prostate cell proliferation
  • Tyrosine kinase

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Picascia, A., Stanzione, R., Chieffi, P., Kisslinger, A., Dikic, I., & Tramontano, D. (2002). Proline-rich tyrosine kinase 2 regulates proliferation and differentiation of prostate cells. Molecular and Cellular Endocrinology, 186(1), 81-87.