Prolonged activation of CXCR4 hampers the release-regulating activity of presynaptic NMDA receptors in rat hippocampal synaptosomes

Guendalina Olivero, Francesca Cisani, Matteo Vergassola, Anna Pittaluga

Research output: Contribution to journalArticle

Abstract

We investigated the impact of the prolonged exposure of rat hippocampal synaptosomes to CXCL12 (3 nM) on the NMDA-mediated release of [ 3 H]D-aspartate ([ 3 H]D-Asp) or [ 3 H]noradrenaline ([ 3 H]NA). Synaptosomes were stimulated twice with NMDA/CXCL12 and the amount of the NMDA-evoked tritium release (S1 and S2) quantified to calculate the S2/S1 ratio. The S2/S1 ratio for both transmitters was drastically decreased by 3 nM CXCL12 between the two stimuli (CXCL12-treated synaptosomes) in a AMD3100-sensitive manner. The phosphorylation of the GluN1 subunit in Ser 896 was reduced in CXCL12-treated synaptosomes, while the overall amount of GluN1 and GluN2B proteins as well as the GluN2B insertion in synaptosomal plasmamembranes were unchanged. We conclude that the CXCR4/NMDA cross-talk is dynamically regulated by the time of activation of the CXCR4s. Our results unveil a functional cross-talk that might account for the severe impairments of central transmission that develop in pathological conditions characterized by CXCL12 overproduction.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalNeurochemistry International
Volume126
DOIs
Publication statusPublished - Jun 1 2019

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Presynaptic Receptors
Synaptosomes
N-Methylaspartate
N-Methyl-D-Aspartate Receptors
D-Aspartic Acid
Tritium
Norepinephrine
Phosphorylation
Proteins

Keywords

  • CXCL12
  • CXCR4
  • Glutamate
  • NMDA receptor
  • Noradrenaline
  • Phospho-GluN1 (Ser896)

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Prolonged activation of CXCR4 hampers the release-regulating activity of presynaptic NMDA receptors in rat hippocampal synaptosomes. / Olivero, Guendalina; Cisani, Francesca; Vergassola, Matteo; Pittaluga, Anna.

In: Neurochemistry International, Vol. 126, 01.06.2019, p. 59-63.

Research output: Contribution to journalArticle

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