Prolonged exposure to particulate pollution, genes associated with glutathione pathways, and DNA methylation in a cohort of older men

Jaime Madrigano, Andrea Baccarelli, Murray A. Mittleman, Robert O. Wright, David Sparrow, Pantel S. Vokonas, Letizia Tarantini, Joel Schwartz

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Background: DNA methylation is a potential pathway linking environmental exposures to disease. Exposure to particulate air pollution has been associated with increased cardiovascular morbidity and mortality, and lower blood DNA methylation has been found in processes related to cardiovascular morbidity. Objective: We hypothesized that prolonged exposure to particulate pollution would be associated with hypomethylation of repetitive DNA elements and that this association would be modified by genes involved in glutathione metabolism and other host characteristics. Methods: DNA methylation of the long interspersed nucleotide element-1 (LINE-1) and the short interspersed nucleotide element Alu were measured by quantitative polymerase chain reaction pyrosequencing in 1,406 blood samples from 706 elderly participants in the Normative Aging Study. We estimated changes in repetitive element DNA methylation associated with ambient particles (particulate matter ≤ 2.5 μm in aerodynamic diameter), black carbon (BC), and sulfates (SO4), with mixed models. We examined multiple exposure windows (1-6 months) before DNA methylation measurement. We investigated whether this association was modified by genotype and phenotype. Results: An interquartile range (IQR) increase in BC over a 90-day period was associated with a decrease of 0.31% 5-methylcytosine (5mC) (95% confidence interval, 0.12-0.50%) in Alu. An IQR increase in SO4 over a 90-day period was associated with a decrease of 0.27% 5mC (0.02-0.52%) in LINE-1. The glutathione S-transferase mu-1-null genotype strengthened the association between BC and Alu hypomethylation. Conclusion: Prolonged exposure to BC and SO4 particles was associated with hypomethylation of two types of repetitive elements.

Original languageEnglish
Pages (from-to)977-982
Number of pages6
JournalEnvironmental Health Perspectives
Volume119
Issue number7
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Soot
DNA Methylation
Glutathione
Long Interspersed Nucleotide Elements
5-Methylcytosine
Genes
Glutathione Transferase
Short Interspersed Nucleotide Elements
Genotype
Morbidity
Particulate Matter
Air Pollution
Environmental Exposure
Sulfates
Confidence Intervals
Phenotype
Polymerase Chain Reaction
Mortality
DNA

Keywords

  • Air pollution
  • DNA methylation
  • Epigenetics
  • Gene-environment

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health

Cite this

Madrigano, J., Baccarelli, A., Mittleman, M. A., Wright, R. O., Sparrow, D., Vokonas, P. S., ... Schwartz, J. (2011). Prolonged exposure to particulate pollution, genes associated with glutathione pathways, and DNA methylation in a cohort of older men. Environmental Health Perspectives, 119(7), 977-982. https://doi.org/10.1289/ehp.1002773

Prolonged exposure to particulate pollution, genes associated with glutathione pathways, and DNA methylation in a cohort of older men. / Madrigano, Jaime; Baccarelli, Andrea; Mittleman, Murray A.; Wright, Robert O.; Sparrow, David; Vokonas, Pantel S.; Tarantini, Letizia; Schwartz, Joel.

In: Environmental Health Perspectives, Vol. 119, No. 7, 07.2011, p. 977-982.

Research output: Contribution to journalArticle

Madrigano, Jaime ; Baccarelli, Andrea ; Mittleman, Murray A. ; Wright, Robert O. ; Sparrow, David ; Vokonas, Pantel S. ; Tarantini, Letizia ; Schwartz, Joel. / Prolonged exposure to particulate pollution, genes associated with glutathione pathways, and DNA methylation in a cohort of older men. In: Environmental Health Perspectives. 2011 ; Vol. 119, No. 7. pp. 977-982.
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