Prolonged voluntary running negatively affects survival and disease prognosis of male SOD1G93A low-copy transgenic mice

Luciana Garbugino, Elisabetta Golini, Alessandro Giuliani, Silvia Mandillo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Amyotrophic Lateral Sclerosis (ALS) is a disease in which physical activity plays a controversial role. Epidemiological studies indicate an association between intense exercise and risk of developing ALS. To study the impact of physical activity on ALS, mouse models rely mostly on forced exercise. In this study we hypothesized that voluntary wheel running could represent a better model of the influence of exercise in the pathogenesis of ALS. We used an automated home-cage running-wheel system that enables individual monitoring of performance. To verify the effect of voluntary running on disease progression, prognosis and survival as well as motor functions, we challenged SOD1G93A low-copy male and female mice on one (1 RW, at age 24 weeks) or multiple (3 RW) running sessions at age 13, 18, and 24 weeks. In parallel we measured performance on Rotarod and Grip strength tests at different ages. Several parameters were analyzed through Principal Component Analysis in order to detect what indices correlate and may be useful for deeper understanding of the relation between exercise and disease development. We found mutant male mice more negatively affected than females by prolonged and repeated exercise. SOD1G93A low-copy male mice showed shorter survival, increased body weight loss and poorer disease prognosis when exposed to multiple running sessions. These findings could encourage the investigation of the pathogenetic mechanisms underlying the supposedly increased risk to develop ALS in humans engaged in specific and intense exercise activities.

Original languageEnglish
Article number275
JournalFrontiers in Behavioral Neuroscience
Volume12
DOIs
Publication statusPublished - Nov 13 2018

Fingerprint

Amyotrophic Lateral Sclerosis
Running
Transgenic Mice
Survival
Hand Strength
Principal Component Analysis
Disease Progression
Weight Loss
Epidemiologic Studies
Body Weight

Keywords

  • ALS
  • Exercise
  • Principal Component Analysis
  • Rotarod
  • Running wheels
  • Sex differences

ASJC Scopus subject areas

  • Neuropsychology and Physiological Psychology
  • Cognitive Neuroscience
  • Behavioral Neuroscience

Cite this

Prolonged voluntary running negatively affects survival and disease prognosis of male SOD1G93A low-copy transgenic mice. / Garbugino, Luciana; Golini, Elisabetta; Giuliani, Alessandro; Mandillo, Silvia.

In: Frontiers in Behavioral Neuroscience, Vol. 12, 275, 13.11.2018.

Research output: Contribution to journalArticle

@article{28fc27af9354473fa7069ef2183d172f,
title = "Prolonged voluntary running negatively affects survival and disease prognosis of male SOD1G93A low-copy transgenic mice",
abstract = "Amyotrophic Lateral Sclerosis (ALS) is a disease in which physical activity plays a controversial role. Epidemiological studies indicate an association between intense exercise and risk of developing ALS. To study the impact of physical activity on ALS, mouse models rely mostly on forced exercise. In this study we hypothesized that voluntary wheel running could represent a better model of the influence of exercise in the pathogenesis of ALS. We used an automated home-cage running-wheel system that enables individual monitoring of performance. To verify the effect of voluntary running on disease progression, prognosis and survival as well as motor functions, we challenged SOD1G93A low-copy male and female mice on one (1 RW, at age 24 weeks) or multiple (3 RW) running sessions at age 13, 18, and 24 weeks. In parallel we measured performance on Rotarod and Grip strength tests at different ages. Several parameters were analyzed through Principal Component Analysis in order to detect what indices correlate and may be useful for deeper understanding of the relation between exercise and disease development. We found mutant male mice more negatively affected than females by prolonged and repeated exercise. SOD1G93A low-copy male mice showed shorter survival, increased body weight loss and poorer disease prognosis when exposed to multiple running sessions. These findings could encourage the investigation of the pathogenetic mechanisms underlying the supposedly increased risk to develop ALS in humans engaged in specific and intense exercise activities.",
keywords = "ALS, Exercise, Principal Component Analysis, Rotarod, Running wheels, Sex differences",
author = "Luciana Garbugino and Elisabetta Golini and Alessandro Giuliani and Silvia Mandillo",
year = "2018",
month = "11",
day = "13",
doi = "10.3389/fnbeh.2018.00275",
language = "English",
volume = "12",
journal = "Frontiers in Behavioral Neuroscience",
issn = "1662-5153",
publisher = "Frontiers Research Foundation",

}

TY - JOUR

T1 - Prolonged voluntary running negatively affects survival and disease prognosis of male SOD1G93A low-copy transgenic mice

AU - Garbugino, Luciana

AU - Golini, Elisabetta

AU - Giuliani, Alessandro

AU - Mandillo, Silvia

PY - 2018/11/13

Y1 - 2018/11/13

N2 - Amyotrophic Lateral Sclerosis (ALS) is a disease in which physical activity plays a controversial role. Epidemiological studies indicate an association between intense exercise and risk of developing ALS. To study the impact of physical activity on ALS, mouse models rely mostly on forced exercise. In this study we hypothesized that voluntary wheel running could represent a better model of the influence of exercise in the pathogenesis of ALS. We used an automated home-cage running-wheel system that enables individual monitoring of performance. To verify the effect of voluntary running on disease progression, prognosis and survival as well as motor functions, we challenged SOD1G93A low-copy male and female mice on one (1 RW, at age 24 weeks) or multiple (3 RW) running sessions at age 13, 18, and 24 weeks. In parallel we measured performance on Rotarod and Grip strength tests at different ages. Several parameters were analyzed through Principal Component Analysis in order to detect what indices correlate and may be useful for deeper understanding of the relation between exercise and disease development. We found mutant male mice more negatively affected than females by prolonged and repeated exercise. SOD1G93A low-copy male mice showed shorter survival, increased body weight loss and poorer disease prognosis when exposed to multiple running sessions. These findings could encourage the investigation of the pathogenetic mechanisms underlying the supposedly increased risk to develop ALS in humans engaged in specific and intense exercise activities.

AB - Amyotrophic Lateral Sclerosis (ALS) is a disease in which physical activity plays a controversial role. Epidemiological studies indicate an association between intense exercise and risk of developing ALS. To study the impact of physical activity on ALS, mouse models rely mostly on forced exercise. In this study we hypothesized that voluntary wheel running could represent a better model of the influence of exercise in the pathogenesis of ALS. We used an automated home-cage running-wheel system that enables individual monitoring of performance. To verify the effect of voluntary running on disease progression, prognosis and survival as well as motor functions, we challenged SOD1G93A low-copy male and female mice on one (1 RW, at age 24 weeks) or multiple (3 RW) running sessions at age 13, 18, and 24 weeks. In parallel we measured performance on Rotarod and Grip strength tests at different ages. Several parameters were analyzed through Principal Component Analysis in order to detect what indices correlate and may be useful for deeper understanding of the relation between exercise and disease development. We found mutant male mice more negatively affected than females by prolonged and repeated exercise. SOD1G93A low-copy male mice showed shorter survival, increased body weight loss and poorer disease prognosis when exposed to multiple running sessions. These findings could encourage the investigation of the pathogenetic mechanisms underlying the supposedly increased risk to develop ALS in humans engaged in specific and intense exercise activities.

KW - ALS

KW - Exercise

KW - Principal Component Analysis

KW - Rotarod

KW - Running wheels

KW - Sex differences

UR - http://www.scopus.com/inward/record.url?scp=85056875150&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056875150&partnerID=8YFLogxK

U2 - 10.3389/fnbeh.2018.00275

DO - 10.3389/fnbeh.2018.00275

M3 - Article

AN - SCOPUS:85056875150

VL - 12

JO - Frontiers in Behavioral Neuroscience

JF - Frontiers in Behavioral Neuroscience

SN - 1662-5153

M1 - 275

ER -