Promoter characterization and structure of the gene encoding mouse lysosomal α-D-mannosidase

S. Stinchi, An Orlacchio, E. Costanzi, J. L. Stirling, A. R. Menghini, A. Orlacchio, Tommaso Beccari

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Mouse lysosomal α-D-mannosidase (EC 3.2.1.24) is an enzyme involved in the catabolism of N-linked glycoproteins. The gene is differentially expressed in mouse tissues, and the highest level of mRNA is found in the epididymis. The expression of mannosidase in the epididymis may be hormonally regulated, since its activity increases with age. To understand the factors affecting the expression of mouse mannosidase, we isolated and characterized the promoter and determined the exon-intron structure. The gene is about 15 kb, consists of 24 exons, and the 5' flanking region contains GC-rich regions, TATA boxes, CAAT boxes, and putative binding sites for the transcription factors Sp1, AP2, and PEA3. PEA3 factor may participate in the transcriptional control of mannosidase expression in the mouse epididymis. In fact, it has been demonstrated that the PEA3 motif is spatially and temporally expressed within the mouse epididymis, and its accumulation is controlled by androgens and testicular factors. A 1279-bp fragment from the initiation codon had the strongest promoter activity, and three different transcription start sites were identified at positions -131, -149, and -174.

Original languageEnglish
Pages (from-to)869-873
Number of pages5
JournalMammalian Genome
Volume9
Issue number11
DOIs
Publication statusPublished - 1998

Fingerprint

Mannosidases
Epididymis
Genes
Exons
Transcription Factor AP-2
GC Rich Sequence
TATA Box
Initiator Codon
5' Flanking Region
Transcription Initiation Site
Introns
Androgens
Glycoproteins
Binding Sites
Messenger RNA
Enzymes

ASJC Scopus subject areas

  • Genetics

Cite this

Stinchi, S., Orlacchio, A., Costanzi, E., Stirling, J. L., Menghini, A. R., Orlacchio, A., & Beccari, T. (1998). Promoter characterization and structure of the gene encoding mouse lysosomal α-D-mannosidase. Mammalian Genome, 9(11), 869-873. https://doi.org/10.1007/s003359900885

Promoter characterization and structure of the gene encoding mouse lysosomal α-D-mannosidase. / Stinchi, S.; Orlacchio, An; Costanzi, E.; Stirling, J. L.; Menghini, A. R.; Orlacchio, A.; Beccari, Tommaso.

In: Mammalian Genome, Vol. 9, No. 11, 1998, p. 869-873.

Research output: Contribution to journalArticle

Stinchi, S, Orlacchio, A, Costanzi, E, Stirling, JL, Menghini, AR, Orlacchio, A & Beccari, T 1998, 'Promoter characterization and structure of the gene encoding mouse lysosomal α-D-mannosidase', Mammalian Genome, vol. 9, no. 11, pp. 869-873. https://doi.org/10.1007/s003359900885
Stinchi, S. ; Orlacchio, An ; Costanzi, E. ; Stirling, J. L. ; Menghini, A. R. ; Orlacchio, A. ; Beccari, Tommaso. / Promoter characterization and structure of the gene encoding mouse lysosomal α-D-mannosidase. In: Mammalian Genome. 1998 ; Vol. 9, No. 11. pp. 869-873.
@article{ffd96448d9844eda9710614df32cee5c,
title = "Promoter characterization and structure of the gene encoding mouse lysosomal α-D-mannosidase",
abstract = "Mouse lysosomal α-D-mannosidase (EC 3.2.1.24) is an enzyme involved in the catabolism of N-linked glycoproteins. The gene is differentially expressed in mouse tissues, and the highest level of mRNA is found in the epididymis. The expression of mannosidase in the epididymis may be hormonally regulated, since its activity increases with age. To understand the factors affecting the expression of mouse mannosidase, we isolated and characterized the promoter and determined the exon-intron structure. The gene is about 15 kb, consists of 24 exons, and the 5' flanking region contains GC-rich regions, TATA boxes, CAAT boxes, and putative binding sites for the transcription factors Sp1, AP2, and PEA3. PEA3 factor may participate in the transcriptional control of mannosidase expression in the mouse epididymis. In fact, it has been demonstrated that the PEA3 motif is spatially and temporally expressed within the mouse epididymis, and its accumulation is controlled by androgens and testicular factors. A 1279-bp fragment from the initiation codon had the strongest promoter activity, and three different transcription start sites were identified at positions -131, -149, and -174.",
author = "S. Stinchi and An Orlacchio and E. Costanzi and Stirling, {J. L.} and Menghini, {A. R.} and A. Orlacchio and Tommaso Beccari",
year = "1998",
doi = "10.1007/s003359900885",
language = "English",
volume = "9",
pages = "869--873",
journal = "Mammalian Genome",
issn = "0938-8990",
publisher = "Springer New York",
number = "11",

}

TY - JOUR

T1 - Promoter characterization and structure of the gene encoding mouse lysosomal α-D-mannosidase

AU - Stinchi, S.

AU - Orlacchio, An

AU - Costanzi, E.

AU - Stirling, J. L.

AU - Menghini, A. R.

AU - Orlacchio, A.

AU - Beccari, Tommaso

PY - 1998

Y1 - 1998

N2 - Mouse lysosomal α-D-mannosidase (EC 3.2.1.24) is an enzyme involved in the catabolism of N-linked glycoproteins. The gene is differentially expressed in mouse tissues, and the highest level of mRNA is found in the epididymis. The expression of mannosidase in the epididymis may be hormonally regulated, since its activity increases with age. To understand the factors affecting the expression of mouse mannosidase, we isolated and characterized the promoter and determined the exon-intron structure. The gene is about 15 kb, consists of 24 exons, and the 5' flanking region contains GC-rich regions, TATA boxes, CAAT boxes, and putative binding sites for the transcription factors Sp1, AP2, and PEA3. PEA3 factor may participate in the transcriptional control of mannosidase expression in the mouse epididymis. In fact, it has been demonstrated that the PEA3 motif is spatially and temporally expressed within the mouse epididymis, and its accumulation is controlled by androgens and testicular factors. A 1279-bp fragment from the initiation codon had the strongest promoter activity, and three different transcription start sites were identified at positions -131, -149, and -174.

AB - Mouse lysosomal α-D-mannosidase (EC 3.2.1.24) is an enzyme involved in the catabolism of N-linked glycoproteins. The gene is differentially expressed in mouse tissues, and the highest level of mRNA is found in the epididymis. The expression of mannosidase in the epididymis may be hormonally regulated, since its activity increases with age. To understand the factors affecting the expression of mouse mannosidase, we isolated and characterized the promoter and determined the exon-intron structure. The gene is about 15 kb, consists of 24 exons, and the 5' flanking region contains GC-rich regions, TATA boxes, CAAT boxes, and putative binding sites for the transcription factors Sp1, AP2, and PEA3. PEA3 factor may participate in the transcriptional control of mannosidase expression in the mouse epididymis. In fact, it has been demonstrated that the PEA3 motif is spatially and temporally expressed within the mouse epididymis, and its accumulation is controlled by androgens and testicular factors. A 1279-bp fragment from the initiation codon had the strongest promoter activity, and three different transcription start sites were identified at positions -131, -149, and -174.

UR - http://www.scopus.com/inward/record.url?scp=0031734838&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031734838&partnerID=8YFLogxK

U2 - 10.1007/s003359900885

DO - 10.1007/s003359900885

M3 - Article

VL - 9

SP - 869

EP - 873

JO - Mammalian Genome

JF - Mammalian Genome

SN - 0938-8990

IS - 11

ER -