Pronatriodilatin gene polymorphisms, microvascular permeability, and diabetic nephropathy in type 1 diabetes mellitus

Monica Nannipieri, Giuseppe Penno, Laura Pucci, Helen Colhoun, Corradino Motti, Anna Bertacca, Loredana Rizzo, Lamberto De Giorgio, Giampaolo Zerbini, Ruggero Mangili, Renzo Navalesi

Research output: Contribution to journalArticle

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Abstract

Approximately 30% of diabetic patients develop nephropathy, the appearance of which is partially under genetic control. Atrial natriuretic peptide (ANP) has associated physiologic effects on the kidney. This study was conducted to examine the relationship between a newly identified and known polymorphism at the pronatriodilatin (PND) gene locus and renal involvement in type 1 diabetic subjects. Of 454 type 1 diabetic patients (219 men, 235 women), 323 showed no sign of nephropathy, 79 had incipient renal involvement, and 52 established nephropathy; 58 healthy control subjects were examined for comparison. Allele frequencies (C 708 versus T 708) were: 0.95 and 0.05 in normoalbuminuric patients, respectively; 0.88 and 0.12 in microalbuminuric patients; 0.96 and 0.04 both in those with overt nephropathy and in healthy control subjects (P = 0.011). Patients with incipient nephropathy were in disequilibrium compared with the total diabetic cohort (P = 0.02). In the same populations, an additional genotype for ScaI polymorphism of the PND gene was tested. The A 1 and A 2 allele frequencies were: 0.21 and 0.79 in normoalbuminuric patients; 0.13 and 0.87 in microalbuminuric patients; 0.06 and 0.94 in type 1 diabetic subjects with overt nephropathy; and 0.20 and 0.80 in healthy control subjects, respectively (P <0.0001). A subset of 55 normotensive patients with type 1 diabetes, well matched for clinical features, plasma ANP levels, and microvascular permeability to macromolecules, was investigated on the basis of the C 708/T and A 2/A 1 polymorphisms. Both transcapillary escape rate of albumin (TER(alb)) and plasma ANP levels were significantly lower in patients with the T 708 than with C 708 allele, as well as in the A 1 than in A 2 allele (TER(alb): T 708 versus C 708:5.5 ± 1.7 versus 7.8 ± 2.0%/h, P = 0.0001; plasma ANP levels: 8.3 ± 3.9 versus 15.3 ± 7.7 pg/ml, P = 0.0003; A 1 versus A 2:6.05 ± 2.2 versus 7.3 ± 2.1%/h, P = 0.044; 8.53 ± 4.6 versus 14.5 ± 7.4 pg/ml, P = 0.0024, respectively). Thus, in a large ethnically homogeneous cohort of diabetic subjects, our data show: (I) a significant association of C 708/T polymorphism with microalbuminuria in long-term diabetes and with both lower plasma ANP levels and widespread albumin leakage; and (2) a strong association between ScaI polymorphism and both diabetic nephropathy and plasma ANP concentrations. These results suggest a possible role of PND gene in conferring protection from nephropathy and microvascular damage in type 1 diabetes.

Original languageEnglish
Pages (from-to)1530-1541
Number of pages12
JournalJournal of the American Society of Nephrology
Volume10
Issue number7
Publication statusPublished - Jul 1999

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Diabetic Nephropathies
Capillary Permeability
Atrial Natriuretic Factor
Type 1 Diabetes Mellitus
Genes
varespladib methyl
Healthy Volunteers
Kidney
Gene Frequency
Albumins
Alleles
Serum Albumin
Genotype

ASJC Scopus subject areas

  • Nephrology

Cite this

Nannipieri, M., Penno, G., Pucci, L., Colhoun, H., Motti, C., Bertacca, A., ... Navalesi, R. (1999). Pronatriodilatin gene polymorphisms, microvascular permeability, and diabetic nephropathy in type 1 diabetes mellitus. Journal of the American Society of Nephrology, 10(7), 1530-1541.

Pronatriodilatin gene polymorphisms, microvascular permeability, and diabetic nephropathy in type 1 diabetes mellitus. / Nannipieri, Monica; Penno, Giuseppe; Pucci, Laura; Colhoun, Helen; Motti, Corradino; Bertacca, Anna; Rizzo, Loredana; De Giorgio, Lamberto; Zerbini, Giampaolo; Mangili, Ruggero; Navalesi, Renzo.

In: Journal of the American Society of Nephrology, Vol. 10, No. 7, 07.1999, p. 1530-1541.

Research output: Contribution to journalArticle

Nannipieri, M, Penno, G, Pucci, L, Colhoun, H, Motti, C, Bertacca, A, Rizzo, L, De Giorgio, L, Zerbini, G, Mangili, R & Navalesi, R 1999, 'Pronatriodilatin gene polymorphisms, microvascular permeability, and diabetic nephropathy in type 1 diabetes mellitus', Journal of the American Society of Nephrology, vol. 10, no. 7, pp. 1530-1541.
Nannipieri, Monica ; Penno, Giuseppe ; Pucci, Laura ; Colhoun, Helen ; Motti, Corradino ; Bertacca, Anna ; Rizzo, Loredana ; De Giorgio, Lamberto ; Zerbini, Giampaolo ; Mangili, Ruggero ; Navalesi, Renzo. / Pronatriodilatin gene polymorphisms, microvascular permeability, and diabetic nephropathy in type 1 diabetes mellitus. In: Journal of the American Society of Nephrology. 1999 ; Vol. 10, No. 7. pp. 1530-1541.
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abstract = "Approximately 30{\%} of diabetic patients develop nephropathy, the appearance of which is partially under genetic control. Atrial natriuretic peptide (ANP) has associated physiologic effects on the kidney. This study was conducted to examine the relationship between a newly identified and known polymorphism at the pronatriodilatin (PND) gene locus and renal involvement in type 1 diabetic subjects. Of 454 type 1 diabetic patients (219 men, 235 women), 323 showed no sign of nephropathy, 79 had incipient renal involvement, and 52 established nephropathy; 58 healthy control subjects were examined for comparison. Allele frequencies (C 708 versus T 708) were: 0.95 and 0.05 in normoalbuminuric patients, respectively; 0.88 and 0.12 in microalbuminuric patients; 0.96 and 0.04 both in those with overt nephropathy and in healthy control subjects (P = 0.011). Patients with incipient nephropathy were in disequilibrium compared with the total diabetic cohort (P = 0.02). In the same populations, an additional genotype for ScaI polymorphism of the PND gene was tested. The A 1 and A 2 allele frequencies were: 0.21 and 0.79 in normoalbuminuric patients; 0.13 and 0.87 in microalbuminuric patients; 0.06 and 0.94 in type 1 diabetic subjects with overt nephropathy; and 0.20 and 0.80 in healthy control subjects, respectively (P <0.0001). A subset of 55 normotensive patients with type 1 diabetes, well matched for clinical features, plasma ANP levels, and microvascular permeability to macromolecules, was investigated on the basis of the C 708/T and A 2/A 1 polymorphisms. Both transcapillary escape rate of albumin (TER(alb)) and plasma ANP levels were significantly lower in patients with the T 708 than with C 708 allele, as well as in the A 1 than in A 2 allele (TER(alb): T 708 versus C 708:5.5 ± 1.7 versus 7.8 ± 2.0{\%}/h, P = 0.0001; plasma ANP levels: 8.3 ± 3.9 versus 15.3 ± 7.7 pg/ml, P = 0.0003; A 1 versus A 2:6.05 ± 2.2 versus 7.3 ± 2.1{\%}/h, P = 0.044; 8.53 ± 4.6 versus 14.5 ± 7.4 pg/ml, P = 0.0024, respectively). Thus, in a large ethnically homogeneous cohort of diabetic subjects, our data show: (I) a significant association of C 708/T polymorphism with microalbuminuria in long-term diabetes and with both lower plasma ANP levels and widespread albumin leakage; and (2) a strong association between ScaI polymorphism and both diabetic nephropathy and plasma ANP concentrations. These results suggest a possible role of PND gene in conferring protection from nephropathy and microvascular damage in type 1 diabetes.",
author = "Monica Nannipieri and Giuseppe Penno and Laura Pucci and Helen Colhoun and Corradino Motti and Anna Bertacca and Loredana Rizzo and {De Giorgio}, Lamberto and Giampaolo Zerbini and Ruggero Mangili and Renzo Navalesi",
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T1 - Pronatriodilatin gene polymorphisms, microvascular permeability, and diabetic nephropathy in type 1 diabetes mellitus

AU - Nannipieri, Monica

AU - Penno, Giuseppe

AU - Pucci, Laura

AU - Colhoun, Helen

AU - Motti, Corradino

AU - Bertacca, Anna

AU - Rizzo, Loredana

AU - De Giorgio, Lamberto

AU - Zerbini, Giampaolo

AU - Mangili, Ruggero

AU - Navalesi, Renzo

PY - 1999/7

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N2 - Approximately 30% of diabetic patients develop nephropathy, the appearance of which is partially under genetic control. Atrial natriuretic peptide (ANP) has associated physiologic effects on the kidney. This study was conducted to examine the relationship between a newly identified and known polymorphism at the pronatriodilatin (PND) gene locus and renal involvement in type 1 diabetic subjects. Of 454 type 1 diabetic patients (219 men, 235 women), 323 showed no sign of nephropathy, 79 had incipient renal involvement, and 52 established nephropathy; 58 healthy control subjects were examined for comparison. Allele frequencies (C 708 versus T 708) were: 0.95 and 0.05 in normoalbuminuric patients, respectively; 0.88 and 0.12 in microalbuminuric patients; 0.96 and 0.04 both in those with overt nephropathy and in healthy control subjects (P = 0.011). Patients with incipient nephropathy were in disequilibrium compared with the total diabetic cohort (P = 0.02). In the same populations, an additional genotype for ScaI polymorphism of the PND gene was tested. The A 1 and A 2 allele frequencies were: 0.21 and 0.79 in normoalbuminuric patients; 0.13 and 0.87 in microalbuminuric patients; 0.06 and 0.94 in type 1 diabetic subjects with overt nephropathy; and 0.20 and 0.80 in healthy control subjects, respectively (P <0.0001). A subset of 55 normotensive patients with type 1 diabetes, well matched for clinical features, plasma ANP levels, and microvascular permeability to macromolecules, was investigated on the basis of the C 708/T and A 2/A 1 polymorphisms. Both transcapillary escape rate of albumin (TER(alb)) and plasma ANP levels were significantly lower in patients with the T 708 than with C 708 allele, as well as in the A 1 than in A 2 allele (TER(alb): T 708 versus C 708:5.5 ± 1.7 versus 7.8 ± 2.0%/h, P = 0.0001; plasma ANP levels: 8.3 ± 3.9 versus 15.3 ± 7.7 pg/ml, P = 0.0003; A 1 versus A 2:6.05 ± 2.2 versus 7.3 ± 2.1%/h, P = 0.044; 8.53 ± 4.6 versus 14.5 ± 7.4 pg/ml, P = 0.0024, respectively). Thus, in a large ethnically homogeneous cohort of diabetic subjects, our data show: (I) a significant association of C 708/T polymorphism with microalbuminuria in long-term diabetes and with both lower plasma ANP levels and widespread albumin leakage; and (2) a strong association between ScaI polymorphism and both diabetic nephropathy and plasma ANP concentrations. These results suggest a possible role of PND gene in conferring protection from nephropathy and microvascular damage in type 1 diabetes.

AB - Approximately 30% of diabetic patients develop nephropathy, the appearance of which is partially under genetic control. Atrial natriuretic peptide (ANP) has associated physiologic effects on the kidney. This study was conducted to examine the relationship between a newly identified and known polymorphism at the pronatriodilatin (PND) gene locus and renal involvement in type 1 diabetic subjects. Of 454 type 1 diabetic patients (219 men, 235 women), 323 showed no sign of nephropathy, 79 had incipient renal involvement, and 52 established nephropathy; 58 healthy control subjects were examined for comparison. Allele frequencies (C 708 versus T 708) were: 0.95 and 0.05 in normoalbuminuric patients, respectively; 0.88 and 0.12 in microalbuminuric patients; 0.96 and 0.04 both in those with overt nephropathy and in healthy control subjects (P = 0.011). Patients with incipient nephropathy were in disequilibrium compared with the total diabetic cohort (P = 0.02). In the same populations, an additional genotype for ScaI polymorphism of the PND gene was tested. The A 1 and A 2 allele frequencies were: 0.21 and 0.79 in normoalbuminuric patients; 0.13 and 0.87 in microalbuminuric patients; 0.06 and 0.94 in type 1 diabetic subjects with overt nephropathy; and 0.20 and 0.80 in healthy control subjects, respectively (P <0.0001). A subset of 55 normotensive patients with type 1 diabetes, well matched for clinical features, plasma ANP levels, and microvascular permeability to macromolecules, was investigated on the basis of the C 708/T and A 2/A 1 polymorphisms. Both transcapillary escape rate of albumin (TER(alb)) and plasma ANP levels were significantly lower in patients with the T 708 than with C 708 allele, as well as in the A 1 than in A 2 allele (TER(alb): T 708 versus C 708:5.5 ± 1.7 versus 7.8 ± 2.0%/h, P = 0.0001; plasma ANP levels: 8.3 ± 3.9 versus 15.3 ± 7.7 pg/ml, P = 0.0003; A 1 versus A 2:6.05 ± 2.2 versus 7.3 ± 2.1%/h, P = 0.044; 8.53 ± 4.6 versus 14.5 ± 7.4 pg/ml, P = 0.0024, respectively). Thus, in a large ethnically homogeneous cohort of diabetic subjects, our data show: (I) a significant association of C 708/T polymorphism with microalbuminuria in long-term diabetes and with both lower plasma ANP levels and widespread albumin leakage; and (2) a strong association between ScaI polymorphism and both diabetic nephropathy and plasma ANP concentrations. These results suggest a possible role of PND gene in conferring protection from nephropathy and microvascular damage in type 1 diabetes.

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