Propagation and properties of Kaposi's sarcoma-derived cell lines obtained from patients with AIDS: Similarity of cultured cells to smooth muscle cells

Alec E. Wittek, Charles D. Mitchell, Gary R. Armstrong, Adriana Albini, George R. Martin, Ruth Seemann, Inessa S. Levenbook, Dale E. Wierenga, Jeanette Ridge, Ruth C. Dunlap, Marilyn L. Lundquist, Ronald G. Steis, Dan L. Longo, Jacqueline Muller, Gerald V. Quinnan

Research output: Contribution to journalArticle

Abstract

Cells derived from Kaposi's sarcoma (KS) were propagated in vitro using conditions which resulted in elimination of contaminating fibroblasts and the emergence of homogeneous cell populations which morphologically resembled smooth muscle cells and had neoplastic characteristics. In long-term culture, they differentiated into large ribbon-like cells with longitudinal fibrillarity of their cytoplasm. These fibrils stained red by Masson trichrome staining, and were reactive with antibodies to desmin. Dense bodies typical of myoblasts were observed in some cells by electron microscopy. The cells did not form capillary structures like endothelial cells, they lacked Weible-Palade bodies, and did not express the blood-clotting Factor VIII-related antigen or receptors for the lectin Ulex europaeus agglutinin I. They did express four other antigens, however, in common with endothelial cells. The cells did not form tumors in athymic nude mice; however, they formed colonies in soft agar, manifested tumor-like growth on muscle organ cultures, and were invasive in an artificial basement membrane invasion assay. The results indicate that a component of KS is closely related to leiomyoblasts and and has neoplastic properties.

Original languageEnglish
Pages (from-to)1485-1493
Number of pages9
JournalAIDS (London, England)
Volume5
Issue number12
Publication statusPublished - Dec 1991

Fingerprint

Kaposi's Sarcoma
Smooth Muscle Myocytes
Cultured Cells
Acquired Immunodeficiency Syndrome
Cell Line
Nude Mice
Endothelial Cells
Artificial Membranes
Antigen Receptors
Blood Coagulation Factors
Desmin
Agglutinins
Myoblasts
Organ Culture Techniques
von Willebrand Factor
Blood Coagulation
Basement Membrane
Agar
Neoplasms
Electron Microscopy

Keywords

  • AIDS
  • Cultivation in vitro
  • Kaposi's sarcoma
  • Smooth muscle cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Wittek, A. E., Mitchell, C. D., Armstrong, G. R., Albini, A., Martin, G. R., Seemann, R., ... Quinnan, G. V. (1991). Propagation and properties of Kaposi's sarcoma-derived cell lines obtained from patients with AIDS: Similarity of cultured cells to smooth muscle cells. AIDS (London, England), 5(12), 1485-1493.

Propagation and properties of Kaposi's sarcoma-derived cell lines obtained from patients with AIDS : Similarity of cultured cells to smooth muscle cells. / Wittek, Alec E.; Mitchell, Charles D.; Armstrong, Gary R.; Albini, Adriana; Martin, George R.; Seemann, Ruth; Levenbook, Inessa S.; Wierenga, Dale E.; Ridge, Jeanette; Dunlap, Ruth C.; Lundquist, Marilyn L.; Steis, Ronald G.; Longo, Dan L.; Muller, Jacqueline; Quinnan, Gerald V.

In: AIDS (London, England), Vol. 5, No. 12, 12.1991, p. 1485-1493.

Research output: Contribution to journalArticle

Wittek, AE, Mitchell, CD, Armstrong, GR, Albini, A, Martin, GR, Seemann, R, Levenbook, IS, Wierenga, DE, Ridge, J, Dunlap, RC, Lundquist, ML, Steis, RG, Longo, DL, Muller, J & Quinnan, GV 1991, 'Propagation and properties of Kaposi's sarcoma-derived cell lines obtained from patients with AIDS: Similarity of cultured cells to smooth muscle cells', AIDS (London, England), vol. 5, no. 12, pp. 1485-1493.
Wittek, Alec E. ; Mitchell, Charles D. ; Armstrong, Gary R. ; Albini, Adriana ; Martin, George R. ; Seemann, Ruth ; Levenbook, Inessa S. ; Wierenga, Dale E. ; Ridge, Jeanette ; Dunlap, Ruth C. ; Lundquist, Marilyn L. ; Steis, Ronald G. ; Longo, Dan L. ; Muller, Jacqueline ; Quinnan, Gerald V. / Propagation and properties of Kaposi's sarcoma-derived cell lines obtained from patients with AIDS : Similarity of cultured cells to smooth muscle cells. In: AIDS (London, England). 1991 ; Vol. 5, No. 12. pp. 1485-1493.
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abstract = "Cells derived from Kaposi's sarcoma (KS) were propagated in vitro using conditions which resulted in elimination of contaminating fibroblasts and the emergence of homogeneous cell populations which morphologically resembled smooth muscle cells and had neoplastic characteristics. In long-term culture, they differentiated into large ribbon-like cells with longitudinal fibrillarity of their cytoplasm. These fibrils stained red by Masson trichrome staining, and were reactive with antibodies to desmin. Dense bodies typical of myoblasts were observed in some cells by electron microscopy. The cells did not form capillary structures like endothelial cells, they lacked Weible-Palade bodies, and did not express the blood-clotting Factor VIII-related antigen or receptors for the lectin Ulex europaeus agglutinin I. They did express four other antigens, however, in common with endothelial cells. The cells did not form tumors in athymic nude mice; however, they formed colonies in soft agar, manifested tumor-like growth on muscle organ cultures, and were invasive in an artificial basement membrane invasion assay. The results indicate that a component of KS is closely related to leiomyoblasts and and has neoplastic properties.",
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