Properties of a human monoclonal antibody specific for the NS4 region of hepatitis C virus

Claudia de Lalla, Antonella Cerino, Carlo Rosa, Silvia Griva, Fabrizio Bonelli, Mario U. Mondelli

Research output: Contribution to journalArticlepeer-review

Abstract

A human monoclonal IgGl(κ) antibody (hmAb) specific for a sequential epitope comprised within the 5-1-1 fragment of the NS4 region of hepatitis C virus (HCV) has been recently generated (1). In this study, B-cell clone supernatant containing the hmAb was purified by passage over a protein A affinity column. Preincubation with synthetic oligopeptides containing the epitope recognized by the hmAb resulted in complete inhibition of binding to the whole recombinant protein, attesting to its specificity. Calculation of the dissociation constant (Kd) using a synthetic 20-mer as antigen gave a value of 3.3372 × 10-8 M, consistent with that of a human IgG. We predict that this hmAb will be helpful in characterizing the as yet unidentified native NS4 protein of HCV.

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalJournal of Hepatology
Volume18
Issue number2
DOIs
Publication statusPublished - 1993

Keywords

  • Dissociation constant
  • Hepatitis C virus
  • Human monoclonal antibody
  • NS4 region

ASJC Scopus subject areas

  • Gastroenterology

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