Properties of a U1 RNA enhancer-like sequence

Gennaro Ciliberto, Franco Palla, Graham Tebb, Iain W. Mattaj, Lannart Philipson

Research output: Contribution to journalArticle

Abstract

The properties of a X.laevis U1B snRNA gene enhancer have been studied by microinjection in Xenopus oocytes. The enhancer-like sequence, defined as a short DNA stretch that is able to activate transcription in an orientation independent manner, is interchangeable between different U snRNA genes. The enhancer sequence alone does not, however, efficiently activate transcription from an SV40 pol II promoter but regains its activity when combined with the U-gene specific proximal sequence element. DNase I rotection experiments show that the X.laevis U1B enhancer can interact specifically with a nuclear factor present in mammalian cells.

Original languageEnglish
Pages (from-to)2403-2416
Number of pages14
JournalNucleic Acids Research
Volume15
Issue number6
DOIs
Publication statusPublished - Mar 25 1987

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

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  • Cite this

    Ciliberto, G., Palla, F., Tebb, G., Mattaj, I. W., & Philipson, L. (1987). Properties of a U1 RNA enhancer-like sequence. Nucleic Acids Research, 15(6), 2403-2416. https://doi.org/10.1093/nar/15.6.2403