Properties of a U1 RNA enhancer-like sequence

Gennaro Ciliberto, Franco Palla, Graham Tebb, Iain W. Mattaj, Lannart Philipson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The properties of a X.laevis U1B snRNA gene enhancer have been studied by microinjection in Xenopus oocytes. The enhancer-like sequence, defined as a short DNA stretch that is able to activate transcription in an orientation independent manner, is interchangeable between different U snRNA genes. The enhancer sequence alone does not, however, efficiently activate transcription from an SV40 pol II promoter but regains its activity when combined with the U-gene specific proximal sequence element. DNase I rotection experiments show that the X.laevis U1B enhancer can interact specifically with a nuclear factor present in mammalian cells.

Original languageEnglish
Pages (from-to)2403-2416
Number of pages14
JournalNucleic Acids Research
Volume15
Issue number6
DOIs
Publication statusPublished - Mar 25 1987

Fingerprint

RNA
Small Nuclear RNA
Genes
Transcription
Gene
Regain
Oocyte
Deoxyribonuclease I
Microinjections
Stretch
Xenopus
Promoter
Oocytes
DNA
Cells
Cell
Experiment
U1 small nuclear RNA
Experiments

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Ciliberto, G., Palla, F., Tebb, G., Mattaj, I. W., & Philipson, L. (1987). Properties of a U1 RNA enhancer-like sequence. Nucleic Acids Research, 15(6), 2403-2416. https://doi.org/10.1093/nar/15.6.2403

Properties of a U1 RNA enhancer-like sequence. / Ciliberto, Gennaro; Palla, Franco; Tebb, Graham; Mattaj, Iain W.; Philipson, Lannart.

In: Nucleic Acids Research, Vol. 15, No. 6, 25.03.1987, p. 2403-2416.

Research output: Contribution to journalArticle

Ciliberto, G, Palla, F, Tebb, G, Mattaj, IW & Philipson, L 1987, 'Properties of a U1 RNA enhancer-like sequence', Nucleic Acids Research, vol. 15, no. 6, pp. 2403-2416. https://doi.org/10.1093/nar/15.6.2403
Ciliberto, Gennaro ; Palla, Franco ; Tebb, Graham ; Mattaj, Iain W. ; Philipson, Lannart. / Properties of a U1 RNA enhancer-like sequence. In: Nucleic Acids Research. 1987 ; Vol. 15, No. 6. pp. 2403-2416.
@article{ecc94b35444d4932a410472fd88d5ff8,
title = "Properties of a U1 RNA enhancer-like sequence",
abstract = "The properties of a X.laevis U1B snRNA gene enhancer have been studied by microinjection in Xenopus oocytes. The enhancer-like sequence, defined as a short DNA stretch that is able to activate transcription in an orientation independent manner, is interchangeable between different U snRNA genes. The enhancer sequence alone does not, however, efficiently activate transcription from an SV40 pol II promoter but regains its activity when combined with the U-gene specific proximal sequence element. DNase I rotection experiments show that the X.laevis U1B enhancer can interact specifically with a nuclear factor present in mammalian cells.",
author = "Gennaro Ciliberto and Franco Palla and Graham Tebb and Mattaj, {Iain W.} and Lannart Philipson",
year = "1987",
month = "3",
day = "25",
doi = "10.1093/nar/15.6.2403",
language = "English",
volume = "15",
pages = "2403--2416",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = ". This work is written by (a) US Government employee(s) and is in the public domain in the US",
number = "6",

}

TY - JOUR

T1 - Properties of a U1 RNA enhancer-like sequence

AU - Ciliberto, Gennaro

AU - Palla, Franco

AU - Tebb, Graham

AU - Mattaj, Iain W.

AU - Philipson, Lannart

PY - 1987/3/25

Y1 - 1987/3/25

N2 - The properties of a X.laevis U1B snRNA gene enhancer have been studied by microinjection in Xenopus oocytes. The enhancer-like sequence, defined as a short DNA stretch that is able to activate transcription in an orientation independent manner, is interchangeable between different U snRNA genes. The enhancer sequence alone does not, however, efficiently activate transcription from an SV40 pol II promoter but regains its activity when combined with the U-gene specific proximal sequence element. DNase I rotection experiments show that the X.laevis U1B enhancer can interact specifically with a nuclear factor present in mammalian cells.

AB - The properties of a X.laevis U1B snRNA gene enhancer have been studied by microinjection in Xenopus oocytes. The enhancer-like sequence, defined as a short DNA stretch that is able to activate transcription in an orientation independent manner, is interchangeable between different U snRNA genes. The enhancer sequence alone does not, however, efficiently activate transcription from an SV40 pol II promoter but regains its activity when combined with the U-gene specific proximal sequence element. DNase I rotection experiments show that the X.laevis U1B enhancer can interact specifically with a nuclear factor present in mammalian cells.

UR - http://www.scopus.com/inward/record.url?scp=0023664196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023664196&partnerID=8YFLogxK

U2 - 10.1093/nar/15.6.2403

DO - 10.1093/nar/15.6.2403

M3 - Article

C2 - 3031597

AN - SCOPUS:0023664196

VL - 15

SP - 2403

EP - 2416

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 6

ER -