Abstract
The distribution and biophysical properties of acetylcholine receptors were studied, using morphological and patch-clamp techniques, in adult rat skeletal muscle fibers dissociated by collagenase and maintained in culture. Up to ten hours after dissociation, there were no changes in either the distribution or the biophysical properties of junctional acetylcholine receptors. In long-term culture (5 to 14 days), a new type of acetylcholine receptor was inserted all over the muscle fibers; the channel properties were characterized by a longer open time and a smaller conductance, similar to what has been observed in in vivo denervated muscles. Using autoradiography, we found that during culture an impaired incorporation of new acetylcholine receptors in the former endplates caused a progressive decrease in the density of junctional acetylcholine receptors. This contrasts with muscle fibers denervated in vivo, where the density of receptors does not change after denervation.
| Original language | English |
|---|---|
| Pages (from-to) | 31-42 |
| Number of pages | 12 |
| Journal | Journal of Membrane Biology |
| Volume | 136 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Oct 1993 |
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Keywords
- Acetylcholine channels
- Denervation
- Patch clamp
- Videoimaging
ASJC Scopus subject areas
- Physiology
- Cell Biology
- Biophysics
Cite this
Properties of acetylcholine receptors in adult rat skeletal muscle fibers in culture. / Grohovaz, Fabio; Lorenzon, Paola; Ruzzier, Fabio; Zorec, Robert.
In: Journal of Membrane Biology, Vol. 136, No. 1, 10.1993, p. 31-42.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Properties of acetylcholine receptors in adult rat skeletal muscle fibers in culture
AU - Grohovaz, Fabio
AU - Lorenzon, Paola
AU - Ruzzier, Fabio
AU - Zorec, Robert
PY - 1993/10
Y1 - 1993/10
N2 - The distribution and biophysical properties of acetylcholine receptors were studied, using morphological and patch-clamp techniques, in adult rat skeletal muscle fibers dissociated by collagenase and maintained in culture. Up to ten hours after dissociation, there were no changes in either the distribution or the biophysical properties of junctional acetylcholine receptors. In long-term culture (5 to 14 days), a new type of acetylcholine receptor was inserted all over the muscle fibers; the channel properties were characterized by a longer open time and a smaller conductance, similar to what has been observed in in vivo denervated muscles. Using autoradiography, we found that during culture an impaired incorporation of new acetylcholine receptors in the former endplates caused a progressive decrease in the density of junctional acetylcholine receptors. This contrasts with muscle fibers denervated in vivo, where the density of receptors does not change after denervation.
AB - The distribution and biophysical properties of acetylcholine receptors were studied, using morphological and patch-clamp techniques, in adult rat skeletal muscle fibers dissociated by collagenase and maintained in culture. Up to ten hours after dissociation, there were no changes in either the distribution or the biophysical properties of junctional acetylcholine receptors. In long-term culture (5 to 14 days), a new type of acetylcholine receptor was inserted all over the muscle fibers; the channel properties were characterized by a longer open time and a smaller conductance, similar to what has been observed in in vivo denervated muscles. Using autoradiography, we found that during culture an impaired incorporation of new acetylcholine receptors in the former endplates caused a progressive decrease in the density of junctional acetylcholine receptors. This contrasts with muscle fibers denervated in vivo, where the density of receptors does not change after denervation.
KW - Acetylcholine channels
KW - Denervation
KW - Patch clamp
KW - Videoimaging
UR - http://www.scopus.com/inward/record.url?scp=0027385355&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027385355&partnerID=8YFLogxK
U2 - 10.1007/BF00241487
DO - 10.1007/BF00241487
M3 - Article
C2 - 8271271
AN - SCOPUS:0027385355
VL - 136
SP - 31
EP - 42
JO - Journal of Membrane Biology
JF - Journal of Membrane Biology
SN - 0022-2631
IS - 1
ER -