Properties of dopaminergic neurons in organotypic mesencephalic-striatal co-cultures - evidence for a facilitatory effect of dopamine on the glutamatergic input mediated by α-1 adrenergic receptors

Maria L. Cucchiaroni, Peter S. Freestone, Nicola Berretta, Maria T. Viscomi, Elisa Bisicchia, Hideyuki Okano, Marco Molinari, Giorgio Bernardi, Janusz Lipski, Nicola B. Mercuri, Ezia Guatteo

Research output: Contribution to journalArticle

Abstract

Organotypic cultures (OCs) have been widely used to investigate the midbrain dopaminergic system, but only a few studies focused on the functional properties of dopaminergic neurons and their synaptic inputs from dopaminergic and non-dopaminergic neurons also contained in such cultures. In addition, it is not clear whether the culturing process affects the intrinsic neuronal properties and the expression of specific receptors and transporters. We performed patch-clamp recordings from dopaminergic neurons in mesencephalic-striatal co-cultures obtained from transgenic mice expressing green fluorescent protein (GFP) under the tyrosine hydroxylase promoter. Some (10/44) GFP+ neurons displayed a bursting activity that renders the firing of these cells similar to that of the dopaminergic neurons in vivo. The culturing process reduced the hyperpolarization-activated current (Ih) and the expression of D2 receptors. Downregulation of D2 receptor mRNA and protein was confirmed with reverse transcriptase polymerase chain reaction and Western blotting. Immunocytochemistry revealed that many synaptic terminals, most likely originating from dopaminergic neurons, co-expressed the dopamine (DA) transporter and the vesicular glutamate transporter-2, suggesting a co-release of DA and glutamate. Interestingly, exogenous DA decreased glutamate release in young cultures [days in vitro (DIV)2 receptors, while in older cultures (DIV>26) DA increased glutamate release by acting on α-1 adrenoreceptors. The facilitatory effect of DA on glutamatergic transmission to midbrain dopaminergic neurons may be important in conditions when the expression of D2 receptors is compromised, such as long-term treatment with antipsychotic drugs. Our data show that midbrain OCs at DIV>26 may provide a suitable model of such conditions.

Original languageEnglish
Pages (from-to)1622-1636
Number of pages15
JournalEuropean Journal of Neuroscience
Volume33
Issue number9
DOIs
Publication statusPublished - May 2011

Fingerprint

Corpus Striatum
Dopaminergic Neurons
Coculture Techniques
Adrenergic Receptors
Dopamine
Mesencephalon
Glutamic Acid
Green Fluorescent Proteins
Vesicular Glutamate Transport Protein 2
Neurons
Dopamine Plasma Membrane Transport Proteins
Presynaptic Terminals
Tyrosine 3-Monooxygenase
Reverse Transcriptase Polymerase Chain Reaction
Transgenic Mice
Antipsychotic Agents
Down-Regulation
Western Blotting
Immunohistochemistry
Messenger RNA

Keywords

  • D receptors
  • Dopamine
  • GABA
  • Glutamate
  • Patch-clamp technique
  • TH-GFP mouse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Properties of dopaminergic neurons in organotypic mesencephalic-striatal co-cultures - evidence for a facilitatory effect of dopamine on the glutamatergic input mediated by α-1 adrenergic receptors. / Cucchiaroni, Maria L.; Freestone, Peter S.; Berretta, Nicola; Viscomi, Maria T.; Bisicchia, Elisa; Okano, Hideyuki; Molinari, Marco; Bernardi, Giorgio; Lipski, Janusz; Mercuri, Nicola B.; Guatteo, Ezia.

In: European Journal of Neuroscience, Vol. 33, No. 9, 05.2011, p. 1622-1636.

Research output: Contribution to journalArticle

Cucchiaroni, Maria L. ; Freestone, Peter S. ; Berretta, Nicola ; Viscomi, Maria T. ; Bisicchia, Elisa ; Okano, Hideyuki ; Molinari, Marco ; Bernardi, Giorgio ; Lipski, Janusz ; Mercuri, Nicola B. ; Guatteo, Ezia. / Properties of dopaminergic neurons in organotypic mesencephalic-striatal co-cultures - evidence for a facilitatory effect of dopamine on the glutamatergic input mediated by α-1 adrenergic receptors. In: European Journal of Neuroscience. 2011 ; Vol. 33, No. 9. pp. 1622-1636.
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