Properties of the purified elongation factor 2 in the thermoacidophilic archaebacterium Sulfolobus solfataricus.

G. Raimo, M. Masullo, A. Dello Russo, A. Parente, A. Gambacorta, V. Bocchini

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The elongation factor 2 (aEF-2) has been purified to homogeneity from the extreme thermoacidophilic archaebacterium Sulfolobus solfataricus. It is the only target protein which is ADP-ribosylated by diphtheria toxin in presence of NAD and this modification abolishes its property to support poly(Phe) synthesis in vitro. The factor is constituted by a single polypeptide chain with a relative molecular mass of 78,000 and an isoelectric point of 5.9. aEF-2 is resistant to heat denaturation as shown by the fact that its capability to be ADP-ribosylated was only 10% reduced after 4 h treatment at 80 degrees C. Its amino acid composition does not reveal significant differences with that of analogous factors in other sources; nevertheless, the deviation function indicates that aEF-2 is related to Sulfolobus acidocaldarius and eukaryotes EF-2 more than to eubacterial EF-G or other archaebacterial EF-2.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalItalian Journal of Biochemistry
Volume42
Issue number1
Publication statusPublished - Jan 1993

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Sulfolobus solfataricus
Peptide Elongation Factor 2
Archaea
Adenosine Diphosphate
Sulfolobus acidocaldarius
Peptide Elongation Factor G
Diphtheria Toxin
Denaturation
Isoelectric Point
Molecular mass
Eukaryota
NAD
Hot Temperature
Amino Acids
Peptides
Chemical analysis
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Properties of the purified elongation factor 2 in the thermoacidophilic archaebacterium Sulfolobus solfataricus. / Raimo, G.; Masullo, M.; Dello Russo, A.; Parente, A.; Gambacorta, A.; Bocchini, V.

In: Italian Journal of Biochemistry, Vol. 42, No. 1, 01.1993, p. 1-11.

Research output: Contribution to journalArticle

Raimo, G. ; Masullo, M. ; Dello Russo, A. ; Parente, A. ; Gambacorta, A. ; Bocchini, V. / Properties of the purified elongation factor 2 in the thermoacidophilic archaebacterium Sulfolobus solfataricus. In: Italian Journal of Biochemistry. 1993 ; Vol. 42, No. 1. pp. 1-11.
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