Propidium iodide staining of cytoautoradiographic preparations for the simultaneous determination of DNA content and grain count

P. A. Giordano, G. Mazzini, A. Riccardi, C. M. Montecucco, G. Ucci, M. Danova

Research output: Contribution to journalArticle

Abstract

A new method is described for staining cell nuclei with propidium iodide in preparations that have been processed for autoradiography. The permeability of the stripping film to the dye molecule has been studied, as have the staining conditions, in order to optimize the stoichiometry of the DNA-dye interaction. A procedure has also been set up to allow the simultaneous measurement of DNA content and grain count on the same cell.

Original languageEnglish
Pages (from-to)1259-1270
Number of pages12
JournalHistochemical Journal
Volume17
Issue number11
DOIs
Publication statusPublished - Nov 1985

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

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