Prospective isolation of functionally distinct radial glial subtypes-Lineage and transcriptome analysis

Luisa Pinto, Michael T. Mader, Martin Irmler, Marco Gentilini, Federico Santoni, Daniela Drechsel, Robert Blum, Ronny Stahl, Alessandro Bulfone, Paolo Malatesta, Johannes Beckers, Magdalena Götz

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Since the discovery of radial glia as the source of neurons, their heterogeneity in regard to neurogenesis has been described by clonal and time-lapse analysis in vitro. However, the molecular determinants specifying neurogenic radial glia differently from radial glia that mostly self-renew remain ill-defined. Here, we isolated two radial glial subsets that co-exist at mid-neurogenesis in the developing cerebral cortex and their immediate progeny. While one subset generates neurons directly, the other is largely non-neurogenic but also gives rise to Tbr2-positive basal precursors, thereby contributing indirectly to neurogenesis. Isolation of these distinct radial glia subtypes allowed determining interesting differences in their transcriptome. These transcriptomes were also strikingly different from the transcriptome of radial glia isolated at the end of neurogenesis. This analysis therefore identifies, for the first time, the lineage origin of basal progenitors and the molecular differences of this lineage in comparison to directly neurogenic and gliogenic radial glia.

Original languageEnglish
Pages (from-to)15-42
Number of pages28
JournalMolecular and Cellular Neuroscience
Volume38
Issue number1
DOIs
Publication statusPublished - May 2008

Fingerprint

Gene Expression Profiling
Neuroglia
Neurogenesis
Transcriptome
Neurons
Cerebral Cortex

Keywords

  • Basal precursors
  • Cerebral cortex
  • Development
  • Gliogenesis
  • Lineage
  • Neurogenesis
  • Oligodendrogenesis
  • Pax6
  • Radial glia
  • Transcriptome

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Prospective isolation of functionally distinct radial glial subtypes-Lineage and transcriptome analysis. / Pinto, Luisa; Mader, Michael T.; Irmler, Martin; Gentilini, Marco; Santoni, Federico; Drechsel, Daniela; Blum, Robert; Stahl, Ronny; Bulfone, Alessandro; Malatesta, Paolo; Beckers, Johannes; Götz, Magdalena.

In: Molecular and Cellular Neuroscience, Vol. 38, No. 1, 05.2008, p. 15-42.

Research output: Contribution to journalArticle

Pinto, L, Mader, MT, Irmler, M, Gentilini, M, Santoni, F, Drechsel, D, Blum, R, Stahl, R, Bulfone, A, Malatesta, P, Beckers, J & Götz, M 2008, 'Prospective isolation of functionally distinct radial glial subtypes-Lineage and transcriptome analysis', Molecular and Cellular Neuroscience, vol. 38, no. 1, pp. 15-42. https://doi.org/10.1016/j.mcn.2008.01.012
Pinto, Luisa ; Mader, Michael T. ; Irmler, Martin ; Gentilini, Marco ; Santoni, Federico ; Drechsel, Daniela ; Blum, Robert ; Stahl, Ronny ; Bulfone, Alessandro ; Malatesta, Paolo ; Beckers, Johannes ; Götz, Magdalena. / Prospective isolation of functionally distinct radial glial subtypes-Lineage and transcriptome analysis. In: Molecular and Cellular Neuroscience. 2008 ; Vol. 38, No. 1. pp. 15-42.
@article{ef525e9481254e0482d9288a0a1ae0b5,
title = "Prospective isolation of functionally distinct radial glial subtypes-Lineage and transcriptome analysis",
abstract = "Since the discovery of radial glia as the source of neurons, their heterogeneity in regard to neurogenesis has been described by clonal and time-lapse analysis in vitro. However, the molecular determinants specifying neurogenic radial glia differently from radial glia that mostly self-renew remain ill-defined. Here, we isolated two radial glial subsets that co-exist at mid-neurogenesis in the developing cerebral cortex and their immediate progeny. While one subset generates neurons directly, the other is largely non-neurogenic but also gives rise to Tbr2-positive basal precursors, thereby contributing indirectly to neurogenesis. Isolation of these distinct radial glia subtypes allowed determining interesting differences in their transcriptome. These transcriptomes were also strikingly different from the transcriptome of radial glia isolated at the end of neurogenesis. This analysis therefore identifies, for the first time, the lineage origin of basal progenitors and the molecular differences of this lineage in comparison to directly neurogenic and gliogenic radial glia.",
keywords = "Basal precursors, Cerebral cortex, Development, Gliogenesis, Lineage, Neurogenesis, Oligodendrogenesis, Pax6, Radial glia, Transcriptome",
author = "Luisa Pinto and Mader, {Michael T.} and Martin Irmler and Marco Gentilini and Federico Santoni and Daniela Drechsel and Robert Blum and Ronny Stahl and Alessandro Bulfone and Paolo Malatesta and Johannes Beckers and Magdalena G{\"o}tz",
year = "2008",
month = "5",
doi = "10.1016/j.mcn.2008.01.012",
language = "English",
volume = "38",
pages = "15--42",
journal = "Molecular and Cellular Neurosciences",
issn = "1044-7431",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Prospective isolation of functionally distinct radial glial subtypes-Lineage and transcriptome analysis

AU - Pinto, Luisa

AU - Mader, Michael T.

AU - Irmler, Martin

AU - Gentilini, Marco

AU - Santoni, Federico

AU - Drechsel, Daniela

AU - Blum, Robert

AU - Stahl, Ronny

AU - Bulfone, Alessandro

AU - Malatesta, Paolo

AU - Beckers, Johannes

AU - Götz, Magdalena

PY - 2008/5

Y1 - 2008/5

N2 - Since the discovery of radial glia as the source of neurons, their heterogeneity in regard to neurogenesis has been described by clonal and time-lapse analysis in vitro. However, the molecular determinants specifying neurogenic radial glia differently from radial glia that mostly self-renew remain ill-defined. Here, we isolated two radial glial subsets that co-exist at mid-neurogenesis in the developing cerebral cortex and their immediate progeny. While one subset generates neurons directly, the other is largely non-neurogenic but also gives rise to Tbr2-positive basal precursors, thereby contributing indirectly to neurogenesis. Isolation of these distinct radial glia subtypes allowed determining interesting differences in their transcriptome. These transcriptomes were also strikingly different from the transcriptome of radial glia isolated at the end of neurogenesis. This analysis therefore identifies, for the first time, the lineage origin of basal progenitors and the molecular differences of this lineage in comparison to directly neurogenic and gliogenic radial glia.

AB - Since the discovery of radial glia as the source of neurons, their heterogeneity in regard to neurogenesis has been described by clonal and time-lapse analysis in vitro. However, the molecular determinants specifying neurogenic radial glia differently from radial glia that mostly self-renew remain ill-defined. Here, we isolated two radial glial subsets that co-exist at mid-neurogenesis in the developing cerebral cortex and their immediate progeny. While one subset generates neurons directly, the other is largely non-neurogenic but also gives rise to Tbr2-positive basal precursors, thereby contributing indirectly to neurogenesis. Isolation of these distinct radial glia subtypes allowed determining interesting differences in their transcriptome. These transcriptomes were also strikingly different from the transcriptome of radial glia isolated at the end of neurogenesis. This analysis therefore identifies, for the first time, the lineage origin of basal progenitors and the molecular differences of this lineage in comparison to directly neurogenic and gliogenic radial glia.

KW - Basal precursors

KW - Cerebral cortex

KW - Development

KW - Gliogenesis

KW - Lineage

KW - Neurogenesis

KW - Oligodendrogenesis

KW - Pax6

KW - Radial glia

KW - Transcriptome

UR - http://www.scopus.com/inward/record.url?scp=43049119575&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43049119575&partnerID=8YFLogxK

U2 - 10.1016/j.mcn.2008.01.012

DO - 10.1016/j.mcn.2008.01.012

M3 - Article

VL - 38

SP - 15

EP - 42

JO - Molecular and Cellular Neurosciences

JF - Molecular and Cellular Neurosciences

SN - 1044-7431

IS - 1

ER -