Prostaglandin E1-induced deconsolidation of thrombin-activated platelet aggregates I: Ultrastructure-computer image analysis

Leon Salganicoff, Matteo A. Russo, Roger W. Sevy

Research output: Contribution to journalArticlepeer-review

Abstract

We have compared, at an ultrastructural-computer image morphometric level, the relaxation induced by Mg-ethylene-bis-oxyethylenenitrilo- tetracetic acid and prostaglandin E1 on a model of a thrombin-activated platelet aggregate. Mg-ethylene-bis-oxyethylenenitrilo-tetracetic acid produced a small increase of 5.0% of the intercellular space over the control levels, and a decrease of 10.0±1.3% of the cross-sectional area of the platelets, with no apparent cytoskeletal alterations. In contrast, the prostaglandin E1-treated preparation shows a 360% increase in the intercellular space and a decrease of the average platelet cross-sectional area of 30.0±2.0% with marked cytoskeletal alterations. We use the term 'deconsolidation' to describe this effect. The enlargement of the intercellular space allows the observation of two types of contacts: (1) a type S (segmental) complex, of approximately 200-nm length that maintains a narrow interplatelet gap of 20-30 nm, filled with a dense intercellular material, and (2) a type R (reticular) complex, formed by scant focal regions of the plasma membrane from opposing platelets that are connected through a mesh of fibrillar or granular material contained within a variable-size space. We hypothesize that deconsolidation is caused by fluid loss from the platelets into the intercellular space. As a result, platelet volume decreases and intercellular space increases.

Original languageEnglish
Pages (from-to)359-371
Number of pages13
JournalThrombosis Research
Volume94
Issue number6
DOIs
Publication statusPublished - Jun 15 1999

Keywords

  • Consolidation
  • Cyclic adenosine monophosphate
  • Cyclic guanosine monophosphate
  • Mg-ethylene-bis-oxyethylenenitrilo-tetracetic acid
  • Platelet volume

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

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