Prostaglandin E2 induction of binding activity to CRE and AP-2 elements in human T lymphocytes

Ada Micali, Nicola Medici, Antonino Sottile, Mario Venza, Isabella Venza, Vincenzo Nigro, Giovanni Alfredo Puca, Diana Teti

Research output: Contribution to journalArticle

Abstract

Prostaglandins of the E series are immunomodulatory agents which exert inhibitory as well as stimulatory effects on a variety of immune responses. Since it is known that PGE2 is able to increase cAMP levels, we investigated whether it can affect gene expression through the activation of the transcription factors which bind enhancer elements in the promoter regions of cAMP-regulated genes. Using electrophoretic mobility shift assay, we demonstrated that a short treatment of human T lymphocytes with PGE2 induces specific binding activity to CRE and AP-2, but not AP-1, DNA elements. Since the okadaic acid, a potent protein phosphatase inhibitor, prolongs the induction of the binding activity, phosphorylation events are likely to occur. This activity seems to be due to increased cAMP levels because forskolin and IBMX mimic the effects of PGE2. More interestingly, transfection experiments with CRE-CAT plasmide show that PGE2 activates the transcription of a CRE-containing promoter. These data support the positive role for PGE2 on some immune functions.

Original languageEnglish
Pages (from-to)99-105
Number of pages7
JournalCellular Immunology
Volume174
Issue number1
DOIs
Publication statusPublished - Nov 25 1996

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Dinoprostone
T-Lymphocytes
1-Methyl-3-isobutylxanthine
Okadaic Acid
Phosphoprotein Phosphatases
Transcription Factor AP-1
Electrophoretic Mobility Shift Assay
Colforsin
Prostaglandins E
Genetic Promoter Regions
Transfection
Transcription Factors
Phosphorylation
Gene Expression
DNA
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Prostaglandin E2 induction of binding activity to CRE and AP-2 elements in human T lymphocytes. / Micali, Ada; Medici, Nicola; Sottile, Antonino; Venza, Mario; Venza, Isabella; Nigro, Vincenzo; Puca, Giovanni Alfredo; Teti, Diana.

In: Cellular Immunology, Vol. 174, No. 1, 25.11.1996, p. 99-105.

Research output: Contribution to journalArticle

Micali, A, Medici, N, Sottile, A, Venza, M, Venza, I, Nigro, V, Puca, GA & Teti, D 1996, 'Prostaglandin E2 induction of binding activity to CRE and AP-2 elements in human T lymphocytes', Cellular Immunology, vol. 174, no. 1, pp. 99-105. https://doi.org/10.1006/cimm.1996.0298
Micali, Ada ; Medici, Nicola ; Sottile, Antonino ; Venza, Mario ; Venza, Isabella ; Nigro, Vincenzo ; Puca, Giovanni Alfredo ; Teti, Diana. / Prostaglandin E2 induction of binding activity to CRE and AP-2 elements in human T lymphocytes. In: Cellular Immunology. 1996 ; Vol. 174, No. 1. pp. 99-105.
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