Prostate cancer stem cells are targets of both innate and adaptive immunity and elicit tumor-specific immune responses

Elena Jachetti, Stefania Mazzoleni, Matteo Grioni, Alessia Ricupito, Chiara Brambillasca, Luca Generoso, Arianna Calcinotto, Massimo Freschi, Anna Mondino, Rossella Galli, Matteo Bellone

Research output: Contribution to journalArticle

Abstract

According to the cancer stem cell (CSC) theory, therapies that do not target the CSC compartment have limited, if any, chances to eradicate established tumors. While cytotoxic T lymphocytes (CTLs) have the potential to recognize and kill single neoplastic cells within a tissue, whether CSC s can be targeted by the immune system during spontaneous or vaccination-elicited responses is poorly defined. Here, we provide experimental evidence showing that CSC lines established from the prostate of transgenic adenocarcinoma of the mouse prostate (TRAMP) mice expressed prostate cancer-associated antigens, MHC Class I and II molecules as well as ligands for natural killer (NK) cell receptors. Indeed, CSC were targets for both NK cell- and CTL-mediated cytotoxicity, both in vitro and in vivo. The administration of dendritic cells pulsed with irradiated CSC s induced a tumor-specific immune response that was more robust than that induced by dendritic cells pulsed with differentiated tumor cells, delayed tumor growth in mice challenged with prostate CSC s and caused tumor regression in TRAMP mice. Thus, CSC are targeted by both innate and adaptive immune responses and might be exploited for the design of novel immunotherapeutic approaches against cancer.

Original languageEnglish
Article numbere24520
JournalOncoImmunology
Volume2
Issue number5
DOIs
Publication statusPublished - May 2013

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Neoplastic Stem Cells
Adaptive Immunity
Innate Immunity
Prostatic Neoplasms
Neoplasms
Prostate
Cytotoxic T-Lymphocytes
Dendritic Cells
Transgenic Mice
Adenocarcinoma
Natural Killer Cell Receptors
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Cell- and Tissue-Based Therapy
Natural Killer Cells
Immune System
Vaccination
Ligands
Cell Line
Growth

Keywords

  • Cancer stem cells
  • Dendritic cell
  • Prostate cancer
  • Tumor immunity
  • Vaccination

ASJC Scopus subject areas

  • Immunology and Allergy
  • Oncology
  • Immunology

Cite this

Prostate cancer stem cells are targets of both innate and adaptive immunity and elicit tumor-specific immune responses. / Jachetti, Elena; Mazzoleni, Stefania; Grioni, Matteo; Ricupito, Alessia; Brambillasca, Chiara; Generoso, Luca; Calcinotto, Arianna; Freschi, Massimo; Mondino, Anna; Galli, Rossella; Bellone, Matteo.

In: OncoImmunology, Vol. 2, No. 5, e24520, 05.2013.

Research output: Contribution to journalArticle

Jachetti, E, Mazzoleni, S, Grioni, M, Ricupito, A, Brambillasca, C, Generoso, L, Calcinotto, A, Freschi, M, Mondino, A, Galli, R & Bellone, M 2013, 'Prostate cancer stem cells are targets of both innate and adaptive immunity and elicit tumor-specific immune responses', OncoImmunology, vol. 2, no. 5, e24520. https://doi.org/10.4161/onci.24520
Jachetti, Elena ; Mazzoleni, Stefania ; Grioni, Matteo ; Ricupito, Alessia ; Brambillasca, Chiara ; Generoso, Luca ; Calcinotto, Arianna ; Freschi, Massimo ; Mondino, Anna ; Galli, Rossella ; Bellone, Matteo. / Prostate cancer stem cells are targets of both innate and adaptive immunity and elicit tumor-specific immune responses. In: OncoImmunology. 2013 ; Vol. 2, No. 5.
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