Prostate carcinoma and green tea: PSA-triggered basement membrane degradation and MMP-2 activation are inhibited by (-)epigallocatechin-3-gallate

Elga Pezzato, Luigi Sartor, Isabella Dell'Aica, Ruggero Dittadi, Massimo Gion, Claudio Belluco, Mario Lise, Spiridione Garbisa

Research output: Contribution to journalArticle

Abstract

Prostate-specific antigen (PSA) is a serine-protease that, in addition to cleaving semenogelins in the seminal coagulum, is able to cleave extracellular matrix glycoproteins, thereby affecting cell migration and metastasis. We here report some new activities of PSA that deserve careful consideration in the cancer context: degradation of gelatin, degradation of type IV collagen in reconstituted basement membrane (Matrigel) and activation of progelatinase A (MMP-2), but not pro-MMP-9, in a cell-free system. Since consumption of green tea has been reported to lower the risk of prostate cancer, we investigated the effects of the major flavanol of green tea, (-)epigallocatechin-3-gallate (EGCG), on expression and activity of PSA by prostate carcinoma cells. In addition to restraint of PSA expression, EGCG was found to inhibit in a dose-dependent manner all the above PSA activities, at concentrations lower than the cytotoxic serine-protease inhibitor PMSF and close to levels measured in the serum following ingestion of green tea. The activity of PSA was suppressed also by the elastase released by the inflammatory leukocytes. These results highlight new PSA activities, suggest gelatin zymography as a new convenient assay for PSA, propose EGCG as natural inhibitor of prostate carcinoma aggressiveness, but also stimulate further investigation on the role of prostatic inflammation.

Original languageEnglish
Pages (from-to)787-792
Number of pages6
JournalInternational Journal of Cancer
Volume112
Issue number5
DOIs
Publication statusPublished - Dec 10 2004

Fingerprint

Tea
Prostate-Specific Antigen
Matrix Metalloproteinases
Basement Membrane
Prostate
Carcinoma
Gelatin
Serine Proteinase Inhibitors
Collagen Type IV
Cell-Free System
epigallocatechin gallate
Pancreatic Elastase
Serine Proteases
Cell Movement
Extracellular Matrix
Prostatic Neoplasms
Glycoproteins
Leukocytes
Eating
Neoplasm Metastasis

Keywords

  • (-)epigallocatechin-3-gallate
  • Invasion
  • Prostate carcinoma: MMP-2 activation
  • Prostate-specific antigen

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Prostate carcinoma and green tea : PSA-triggered basement membrane degradation and MMP-2 activation are inhibited by (-)epigallocatechin-3-gallate. / Pezzato, Elga; Sartor, Luigi; Dell'Aica, Isabella; Dittadi, Ruggero; Gion, Massimo; Belluco, Claudio; Lise, Mario; Garbisa, Spiridione.

In: International Journal of Cancer, Vol. 112, No. 5, 10.12.2004, p. 787-792.

Research output: Contribution to journalArticle

Pezzato, Elga ; Sartor, Luigi ; Dell'Aica, Isabella ; Dittadi, Ruggero ; Gion, Massimo ; Belluco, Claudio ; Lise, Mario ; Garbisa, Spiridione. / Prostate carcinoma and green tea : PSA-triggered basement membrane degradation and MMP-2 activation are inhibited by (-)epigallocatechin-3-gallate. In: International Journal of Cancer. 2004 ; Vol. 112, No. 5. pp. 787-792.
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