Protection of internal (TTAGGG)n repeats in Chinese hamster cells by telomeric protein TRF1

Raisa Ivanovna Krutilina, Alexandra Nikolaevna Smirnova, Olga Stanislavovna Mudrak, Nadezhda Mikhailovna Pleskach, Maria Pavlovna Svetlova, Shiao Li Oei, Peter M. Yau, Edwin Morton Bradbury, Andrey Olegovich Zalensky, Nikolai Viktorovich Tomilin

Research output: Contribution to journalArticle

Abstract

Chinese hamster cells have large interstitial (TTAGGG) bands (ITs) which are unstable and should be protected by an unknown mechanism. Here, we expressed in Chinese hamster V79 cells green fluorescent protein (GFP)-tagged human TRF1, and found that a major fraction of GFP-TRF1 bound to ITs is diffusionally mobile. This fraction strongly decreases after treatment of cells with wortmannin, a protein kinase inhibitor, and this drug also increases the frequency of chromosome aberrations. Ionizing radiation does not induce detectable translocation of GFP-TRF1 to the sites of random double-strand breaks visualized using antibodies against histone γ-H2AX. TRF1 is known to be eliminated from telomeres by overexpression of tankyrase 1 which induces TRF1 poly(ADP-ribosyl)ation. We transfected V79 cells by plasmid encoding tankyrase 1 and found that the frequency of chromosome rearrangements is increased in these cells independently of their treatment by IR. Taken together, our results suggest that TRF1 is involved in sequence-specific protection of internal nontelomeric (TTAGGG)n repeats.

Original languageEnglish
Pages (from-to)6690-6698
Number of pages9
JournalOncogene
Volume22
Issue number42 REV. ISS. 4
Publication statusPublished - Sep 29 2003

Fingerprint

Telomeric Repeat Binding Protein 1
Cricetulus
Tankyrases
Green Fluorescent Proteins
Telomere
Protein Kinase Inhibitors
Ionizing Radiation
Chromosome Aberrations
Histones
Adenosine Diphosphate
Plasmids
Chromosomes
Antibodies
Pharmaceutical Preparations

Keywords

  • Instability
  • Internal telomeric repeats
  • Tankyrase 1
  • TRF1
  • Wortmannin

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Krutilina, R. I., Smirnova, A. N., Mudrak, O. S., Pleskach, N. M., Svetlova, M. P., Oei, S. L., ... Tomilin, N. V. (2003). Protection of internal (TTAGGG)n repeats in Chinese hamster cells by telomeric protein TRF1. Oncogene, 22(42 REV. ISS. 4), 6690-6698.

Protection of internal (TTAGGG)n repeats in Chinese hamster cells by telomeric protein TRF1. / Krutilina, Raisa Ivanovna; Smirnova, Alexandra Nikolaevna; Mudrak, Olga Stanislavovna; Pleskach, Nadezhda Mikhailovna; Svetlova, Maria Pavlovna; Oei, Shiao Li; Yau, Peter M.; Bradbury, Edwin Morton; Zalensky, Andrey Olegovich; Tomilin, Nikolai Viktorovich.

In: Oncogene, Vol. 22, No. 42 REV. ISS. 4, 29.09.2003, p. 6690-6698.

Research output: Contribution to journalArticle

Krutilina, RI, Smirnova, AN, Mudrak, OS, Pleskach, NM, Svetlova, MP, Oei, SL, Yau, PM, Bradbury, EM, Zalensky, AO & Tomilin, NV 2003, 'Protection of internal (TTAGGG)n repeats in Chinese hamster cells by telomeric protein TRF1', Oncogene, vol. 22, no. 42 REV. ISS. 4, pp. 6690-6698.
Krutilina RI, Smirnova AN, Mudrak OS, Pleskach NM, Svetlova MP, Oei SL et al. Protection of internal (TTAGGG)n repeats in Chinese hamster cells by telomeric protein TRF1. Oncogene. 2003 Sep 29;22(42 REV. ISS. 4):6690-6698.
Krutilina, Raisa Ivanovna ; Smirnova, Alexandra Nikolaevna ; Mudrak, Olga Stanislavovna ; Pleskach, Nadezhda Mikhailovna ; Svetlova, Maria Pavlovna ; Oei, Shiao Li ; Yau, Peter M. ; Bradbury, Edwin Morton ; Zalensky, Andrey Olegovich ; Tomilin, Nikolai Viktorovich. / Protection of internal (TTAGGG)n repeats in Chinese hamster cells by telomeric protein TRF1. In: Oncogene. 2003 ; Vol. 22, No. 42 REV. ISS. 4. pp. 6690-6698.
@article{56e74b112a0349fda8fd90f61d12f571,
title = "Protection of internal (TTAGGG)n repeats in Chinese hamster cells by telomeric protein TRF1",
abstract = "Chinese hamster cells have large interstitial (TTAGGG) bands (ITs) which are unstable and should be protected by an unknown mechanism. Here, we expressed in Chinese hamster V79 cells green fluorescent protein (GFP)-tagged human TRF1, and found that a major fraction of GFP-TRF1 bound to ITs is diffusionally mobile. This fraction strongly decreases after treatment of cells with wortmannin, a protein kinase inhibitor, and this drug also increases the frequency of chromosome aberrations. Ionizing radiation does not induce detectable translocation of GFP-TRF1 to the sites of random double-strand breaks visualized using antibodies against histone γ-H2AX. TRF1 is known to be eliminated from telomeres by overexpression of tankyrase 1 which induces TRF1 poly(ADP-ribosyl)ation. We transfected V79 cells by plasmid encoding tankyrase 1 and found that the frequency of chromosome rearrangements is increased in these cells independently of their treatment by IR. Taken together, our results suggest that TRF1 is involved in sequence-specific protection of internal nontelomeric (TTAGGG)n repeats.",
keywords = "Instability, Internal telomeric repeats, Tankyrase 1, TRF1, Wortmannin",
author = "Krutilina, {Raisa Ivanovna} and Smirnova, {Alexandra Nikolaevna} and Mudrak, {Olga Stanislavovna} and Pleskach, {Nadezhda Mikhailovna} and Svetlova, {Maria Pavlovna} and Oei, {Shiao Li} and Yau, {Peter M.} and Bradbury, {Edwin Morton} and Zalensky, {Andrey Olegovich} and Tomilin, {Nikolai Viktorovich}",
year = "2003",
month = "9",
day = "29",
language = "English",
volume = "22",
pages = "6690--6698",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "42 REV. ISS. 4",

}

TY - JOUR

T1 - Protection of internal (TTAGGG)n repeats in Chinese hamster cells by telomeric protein TRF1

AU - Krutilina, Raisa Ivanovna

AU - Smirnova, Alexandra Nikolaevna

AU - Mudrak, Olga Stanislavovna

AU - Pleskach, Nadezhda Mikhailovna

AU - Svetlova, Maria Pavlovna

AU - Oei, Shiao Li

AU - Yau, Peter M.

AU - Bradbury, Edwin Morton

AU - Zalensky, Andrey Olegovich

AU - Tomilin, Nikolai Viktorovich

PY - 2003/9/29

Y1 - 2003/9/29

N2 - Chinese hamster cells have large interstitial (TTAGGG) bands (ITs) which are unstable and should be protected by an unknown mechanism. Here, we expressed in Chinese hamster V79 cells green fluorescent protein (GFP)-tagged human TRF1, and found that a major fraction of GFP-TRF1 bound to ITs is diffusionally mobile. This fraction strongly decreases after treatment of cells with wortmannin, a protein kinase inhibitor, and this drug also increases the frequency of chromosome aberrations. Ionizing radiation does not induce detectable translocation of GFP-TRF1 to the sites of random double-strand breaks visualized using antibodies against histone γ-H2AX. TRF1 is known to be eliminated from telomeres by overexpression of tankyrase 1 which induces TRF1 poly(ADP-ribosyl)ation. We transfected V79 cells by plasmid encoding tankyrase 1 and found that the frequency of chromosome rearrangements is increased in these cells independently of their treatment by IR. Taken together, our results suggest that TRF1 is involved in sequence-specific protection of internal nontelomeric (TTAGGG)n repeats.

AB - Chinese hamster cells have large interstitial (TTAGGG) bands (ITs) which are unstable and should be protected by an unknown mechanism. Here, we expressed in Chinese hamster V79 cells green fluorescent protein (GFP)-tagged human TRF1, and found that a major fraction of GFP-TRF1 bound to ITs is diffusionally mobile. This fraction strongly decreases after treatment of cells with wortmannin, a protein kinase inhibitor, and this drug also increases the frequency of chromosome aberrations. Ionizing radiation does not induce detectable translocation of GFP-TRF1 to the sites of random double-strand breaks visualized using antibodies against histone γ-H2AX. TRF1 is known to be eliminated from telomeres by overexpression of tankyrase 1 which induces TRF1 poly(ADP-ribosyl)ation. We transfected V79 cells by plasmid encoding tankyrase 1 and found that the frequency of chromosome rearrangements is increased in these cells independently of their treatment by IR. Taken together, our results suggest that TRF1 is involved in sequence-specific protection of internal nontelomeric (TTAGGG)n repeats.

KW - Instability

KW - Internal telomeric repeats

KW - Tankyrase 1

KW - TRF1

KW - Wortmannin

UR - http://www.scopus.com/inward/record.url?scp=0242606276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242606276&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0242606276

VL - 22

SP - 6690

EP - 6698

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 42 REV. ISS. 4

ER -