Protective effect of 3-aminobenzamide, an inhibitor of poly (ADP-ribose) synthetase, against streptozotocin-induced diabetes

P. Masiello, T. Locci Cubeddu, G. Frosina, E. Bergamini

Research output: Contribution to journalArticle

Abstract

The addition of 3-aminobenzamide (a potent inhibitor of poly(ADP-ribose)synthetase) into the incubation medium, prevents streptozotocin-induced inhibition of glucose-stimulated insulin release from isolated islets [control 142±14μU·islet-1·h-1; streptozotocin (0.5mg/ml) 31±8; 3-aminobenzamide (l.0 mg/ml) 96±11; streptozotocin plus 3-aminobenzamide 122±19]. In vivo, intraperitoneal 3-aminobenzamide 300 mg/kg body weight prevents the appearance of overt diabetes in streptozotocin-treated rats. These protective effects of 3-aminobenzamide are dose-dependent and are similar to those exerted by nicotinamide. Taking into account that poly ADP-ribosylation is involved in the repair of damaged DNA, the protection exerted by 3-aminobenzamide against the diabetogenic effect of streptozotocin strongly supports the view that this acute effect may be a major consequence of the activation of DNA repair mechanisms in islet cells.

Original languageEnglish
Pages (from-to)683-686
Number of pages4
JournalDiabetologia
Volume28
Issue number9
DOIs
Publication statusPublished - Sep 1985

Keywords

  • 3-aminobenzamide
  • Experimental diabetes
  • immunoreactive insulin
  • insulin release
  • isolated islets
  • nicotinamide
  • poly(ADP-ibose)synthetase inhibitors
  • streptozotocin

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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