Abstract
In the present study, we show that an aqueous extract of Arbutus unedo L. (AuE), a Mediterranean endemic plant widely employed as an astringent, diuretic and urinary anti-septic, in vitro down-regulates the expression of some inflammatory genes, such as those encoding inducible nitric oxide synthase (iNOS) and intracellular adhesion molecule-(ICAM)-1, exerting a inhibitory action on both IFN-γ-elicited STAT1 activation and IL-6-elicited STAT3 activation. To evaluate further the effect of AuE in animal models of acute inflammation, we examined whether AuE administration attenuates inflammatory response of murine induced by intrapleural injection of carrageenan. For this purpose we studied: (1) STAT1/3 activation, (2) TNF-α, IL-1β and IL-6 production in pleural exudate, (3) lung iNOS, COX-2 and ICAM-1 expression, (4) neutrophil infiltration, (5) the nitration of cellular proteins by peroxynitrite, (6) lipid peroxidation, (7) prostaglandin E2 and nitrite/nitrate levels and (8) lung injury. We show that AuE strongly down-regulates STAT3 activation induced in the lung by carrageenan with concomitant attenuation of all parameters examined associated with inflammation, suggesting that STAT3 should be a new molecular target for anti-inflammatory treatment. This study demonstrates that acute lung inflammation is significantly attenuated by the treatment with AuE.
| Original language | English |
|---|---|
| Pages (from-to) | 110-124 |
| Number of pages | 15 |
| Journal | Pharmacological Research |
| Volume | 57 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2008 |
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Keywords
- Acute inflammation
- Arbutus unedo L. (AuE) extract
- STAT3 activation
ASJC Scopus subject areas
- Pharmacology
Cite this
Protective effect of Arbutus unedo aqueous extract in carrageenan-induced lung inflammation in mice. / Mariotto, Sofia; Esposito, Emanuela; Di Paola, Rosanna; Ciampa, Anna; Mazzon, Emanuela; de Prati, Alessandra Carcereri; Darra, Elena; Vincenzi, Simone; Cucinotta, Giovanni; Caminiti, Rocco; Suzuki, Hisanori; Cuzzocrea, Salvatore.
In: Pharmacological Research, Vol. 57, No. 2, 02.2008, p. 110-124.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protective effect of Arbutus unedo aqueous extract in carrageenan-induced lung inflammation in mice
AU - Mariotto, Sofia
AU - Esposito, Emanuela
AU - Di Paola, Rosanna
AU - Ciampa, Anna
AU - Mazzon, Emanuela
AU - de Prati, Alessandra Carcereri
AU - Darra, Elena
AU - Vincenzi, Simone
AU - Cucinotta, Giovanni
AU - Caminiti, Rocco
AU - Suzuki, Hisanori
AU - Cuzzocrea, Salvatore
PY - 2008/2
Y1 - 2008/2
N2 - In the present study, we show that an aqueous extract of Arbutus unedo L. (AuE), a Mediterranean endemic plant widely employed as an astringent, diuretic and urinary anti-septic, in vitro down-regulates the expression of some inflammatory genes, such as those encoding inducible nitric oxide synthase (iNOS) and intracellular adhesion molecule-(ICAM)-1, exerting a inhibitory action on both IFN-γ-elicited STAT1 activation and IL-6-elicited STAT3 activation. To evaluate further the effect of AuE in animal models of acute inflammation, we examined whether AuE administration attenuates inflammatory response of murine induced by intrapleural injection of carrageenan. For this purpose we studied: (1) STAT1/3 activation, (2) TNF-α, IL-1β and IL-6 production in pleural exudate, (3) lung iNOS, COX-2 and ICAM-1 expression, (4) neutrophil infiltration, (5) the nitration of cellular proteins by peroxynitrite, (6) lipid peroxidation, (7) prostaglandin E2 and nitrite/nitrate levels and (8) lung injury. We show that AuE strongly down-regulates STAT3 activation induced in the lung by carrageenan with concomitant attenuation of all parameters examined associated with inflammation, suggesting that STAT3 should be a new molecular target for anti-inflammatory treatment. This study demonstrates that acute lung inflammation is significantly attenuated by the treatment with AuE.
AB - In the present study, we show that an aqueous extract of Arbutus unedo L. (AuE), a Mediterranean endemic plant widely employed as an astringent, diuretic and urinary anti-septic, in vitro down-regulates the expression of some inflammatory genes, such as those encoding inducible nitric oxide synthase (iNOS) and intracellular adhesion molecule-(ICAM)-1, exerting a inhibitory action on both IFN-γ-elicited STAT1 activation and IL-6-elicited STAT3 activation. To evaluate further the effect of AuE in animal models of acute inflammation, we examined whether AuE administration attenuates inflammatory response of murine induced by intrapleural injection of carrageenan. For this purpose we studied: (1) STAT1/3 activation, (2) TNF-α, IL-1β and IL-6 production in pleural exudate, (3) lung iNOS, COX-2 and ICAM-1 expression, (4) neutrophil infiltration, (5) the nitration of cellular proteins by peroxynitrite, (6) lipid peroxidation, (7) prostaglandin E2 and nitrite/nitrate levels and (8) lung injury. We show that AuE strongly down-regulates STAT3 activation induced in the lung by carrageenan with concomitant attenuation of all parameters examined associated with inflammation, suggesting that STAT3 should be a new molecular target for anti-inflammatory treatment. This study demonstrates that acute lung inflammation is significantly attenuated by the treatment with AuE.
KW - Acute inflammation
KW - Arbutus unedo L. (AuE) extract
KW - STAT3 activation
UR - http://www.scopus.com/inward/record.url?scp=40149094164&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=40149094164&partnerID=8YFLogxK
U2 - 10.1016/j.phrs.2007.12.005
DO - 10.1016/j.phrs.2007.12.005
M3 - Article
C2 - 18249557
AN - SCOPUS:40149094164
VL - 57
SP - 110
EP - 124
JO - Pharmacological Research
JF - Pharmacological Research
SN - 1043-6618
IS - 2
ER -