Protective effect of ischemic preconditioning on the jejunal graft mucosa injury during cold preservation

Zuzana Jonecova, Stefan Toth, Milan Maretta, Rachele Ciccocioppo, Jan Varga, Luis Rodrigo, Peter Kruzliak

Research output: Contribution to journalArticle

Abstract

Protection of intestinal graft mucosa during cold preservation is still an unmet need in clinical practice, thus affecting the success of transplantation. The present study investigates the ability of two ischemic preconditioning (IPC) procedures to limit cold preservation injury. Three groups of Sprague-Dawley rats were recruited (n. =. 11 each) as follows: the short IPC (SIPC) performed through 4. cycles of mesenteric ischemia of 4. min each followed by 10. min of reperfusion, the long IPC (LIPC) obtained by 2 ischemic cycles of 12. min each followed by 10. min of reperfusion, and the control group (C) without IPC. Grafts were then stored in cold histidine-tryptophan-ketoglutarate solution and samples were taken at 0, 3, 6 and 9. h lasting preservation. Both IPC groups showed an advanced degree of preservation with delayed development of graft mucosa damage, mainly in the crypt region. At the beginning of preservation, the graft mucosa in both IPC groups showed lower degree of mucosal injury index (MII) by 50% in comparison with C group. Specifically, a significant improvement of MII was observed after 3. h of preservation in the LIPC group (p

Original languageEnglish
Pages (from-to)229-235
Number of pages7
JournalExperimental and Molecular Pathology
Volume99
Issue number2
DOIs
Publication statusPublished - Oct 1 2015

Keywords

  • Apoptosis
  • Cold preservation
  • Graft mucosa
  • Histopathology
  • Ischemic preconditioning
  • Laminin
  • Small intestine

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine
  • Medicine(all)

Fingerprint Dive into the research topics of 'Protective effect of ischemic preconditioning on the jejunal graft mucosa injury during cold preservation'. Together they form a unique fingerprint.

  • Cite this