Protective effect of ischemic preconditioning on the jejunal graft mucosa injury during cold preservation

Zuzana Jonecova; Stefan Toth; Milan Maretta; Rachele Ciccocioppo; Jan Varga; Luis Rodrigo; Peter Kruzliak

doi:10.1016/j.yexmp.2015.06.020

Protective effect of ischemic preconditioning on the jejunal graft mucosa injury during cold preservation

Zuzana Jonecova, Stefan Toth, Milan Maretta, Rachele Ciccocioppo, Jan Varga, Luis Rodrigo, Peter Kruzliak

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

Protection of intestinal graft mucosa during cold preservation is still an unmet need in clinical practice, thus affecting the success of transplantation. The present study investigates the ability of two ischemic preconditioning (IPC) procedures to limit cold preservation injury. Three groups of Sprague-Dawley rats were recruited (n. =. 11 each) as follows: the short IPC (SIPC) performed through 4. cycles of mesenteric ischemia of 4. min each followed by 10. min of reperfusion, the long IPC (LIPC) obtained by 2 ischemic cycles of 12. min each followed by 10. min of reperfusion, and the control group (C) without IPC. Grafts were then stored in cold histidine-tryptophan-ketoglutarate solution and samples were taken at 0, 3, 6 and 9. h lasting preservation. Both IPC groups showed an advanced degree of preservation with delayed development of graft mucosa damage, mainly in the crypt region. At the beginning of preservation, the graft mucosa in both IPC groups showed lower degree of mucosal injury index (MII) by 50% in comparison with C group. Specifically, a significant improvement of MII was observed after 3. h of preservation in the LIPC group (p

Original language	English
Pages (from-to)	229-235
Number of pages	7
Journal	Experimental and Molecular Pathology
Volume	99
Issue number	2
DOIs	https://doi.org/10.1016/j.yexmp.2015.06.020
Publication status	Published - Oct 1 2015

Keywords

Apoptosis
Cold preservation
Graft mucosa
Histopathology
Ischemic preconditioning
Laminin
Small intestine

ASJC Scopus subject areas

Clinical Biochemistry
Molecular Biology
Pathology and Forensic Medicine
Medicine(all)

Access to Document

10.1016/j.yexmp.2015.06.020

Fingerprint Dive into the research topics of 'Protective effect of ischemic preconditioning on the jejunal graft mucosa injury during cold preservation'. Together they form a unique fingerprint.

Cite this

Protective effect of ischemic preconditioning on the jejunal graft mucosa injury during cold preservation. / Jonecova, Zuzana; Toth, Stefan; Maretta, Milan; Ciccocioppo, Rachele; Varga, Jan; Rodrigo, Luis; Kruzliak, Peter.

In: Experimental and Molecular Pathology, Vol. 99, No. 2, 01.10.2015, p. 229-235.

Research output: Contribution to journal › Article › peer-review

@article{0789379ca2ca492fb9475c57ef87744e,

title = "Protective effect of ischemic preconditioning on the jejunal graft mucosa injury during cold preservation",

abstract = "Protection of intestinal graft mucosa during cold preservation is still an unmet need in clinical practice, thus affecting the success of transplantation. The present study investigates the ability of two ischemic preconditioning (IPC) procedures to limit cold preservation injury. Three groups of Sprague-Dawley rats were recruited (n. =. 11 each) as follows: the short IPC (SIPC) performed through 4. cycles of mesenteric ischemia of 4. min each followed by 10. min of reperfusion, the long IPC (LIPC) obtained by 2 ischemic cycles of 12. min each followed by 10. min of reperfusion, and the control group (C) without IPC. Grafts were then stored in cold histidine-tryptophan-ketoglutarate solution and samples were taken at 0, 3, 6 and 9. h lasting preservation. Both IPC groups showed an advanced degree of preservation with delayed development of graft mucosa damage, mainly in the crypt region. At the beginning of preservation, the graft mucosa in both IPC groups showed lower degree of mucosal injury index (MII) by 50% in comparison with C group. Specifically, a significant improvement of MII was observed after 3. h of preservation in the LIPC group (p",

keywords = "Apoptosis, Cold preservation, Graft mucosa, Histopathology, Ischemic preconditioning, Laminin, Small intestine",

author = "Zuzana Jonecova and Stefan Toth and Milan Maretta and Rachele Ciccocioppo and Jan Varga and Luis Rodrigo and Peter Kruzliak",

year = "2015",

month = oct,

day = "1",

doi = "10.1016/j.yexmp.2015.06.020",

language = "English",

volume = "99",

pages = "229--235",

journal = "Experimental and Molecular Pathology",

issn = "0014-4800",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Protective effect of ischemic preconditioning on the jejunal graft mucosa injury during cold preservation

AU - Jonecova, Zuzana

AU - Toth, Stefan

AU - Maretta, Milan

AU - Ciccocioppo, Rachele

AU - Varga, Jan

AU - Rodrigo, Luis

AU - Kruzliak, Peter

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Protection of intestinal graft mucosa during cold preservation is still an unmet need in clinical practice, thus affecting the success of transplantation. The present study investigates the ability of two ischemic preconditioning (IPC) procedures to limit cold preservation injury. Three groups of Sprague-Dawley rats were recruited (n. =. 11 each) as follows: the short IPC (SIPC) performed through 4. cycles of mesenteric ischemia of 4. min each followed by 10. min of reperfusion, the long IPC (LIPC) obtained by 2 ischemic cycles of 12. min each followed by 10. min of reperfusion, and the control group (C) without IPC. Grafts were then stored in cold histidine-tryptophan-ketoglutarate solution and samples were taken at 0, 3, 6 and 9. h lasting preservation. Both IPC groups showed an advanced degree of preservation with delayed development of graft mucosa damage, mainly in the crypt region. At the beginning of preservation, the graft mucosa in both IPC groups showed lower degree of mucosal injury index (MII) by 50% in comparison with C group. Specifically, a significant improvement of MII was observed after 3. h of preservation in the LIPC group (p

AB - Protection of intestinal graft mucosa during cold preservation is still an unmet need in clinical practice, thus affecting the success of transplantation. The present study investigates the ability of two ischemic preconditioning (IPC) procedures to limit cold preservation injury. Three groups of Sprague-Dawley rats were recruited (n. =. 11 each) as follows: the short IPC (SIPC) performed through 4. cycles of mesenteric ischemia of 4. min each followed by 10. min of reperfusion, the long IPC (LIPC) obtained by 2 ischemic cycles of 12. min each followed by 10. min of reperfusion, and the control group (C) without IPC. Grafts were then stored in cold histidine-tryptophan-ketoglutarate solution and samples were taken at 0, 3, 6 and 9. h lasting preservation. Both IPC groups showed an advanced degree of preservation with delayed development of graft mucosa damage, mainly in the crypt region. At the beginning of preservation, the graft mucosa in both IPC groups showed lower degree of mucosal injury index (MII) by 50% in comparison with C group. Specifically, a significant improvement of MII was observed after 3. h of preservation in the LIPC group (p

KW - Apoptosis

KW - Cold preservation

KW - Graft mucosa

KW - Histopathology

KW - Ischemic preconditioning

KW - Laminin

KW - Small intestine

UR - http://www.scopus.com/inward/record.url?scp=84937911840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937911840&partnerID=8YFLogxK

U2 - 10.1016/j.yexmp.2015.06.020

DO - 10.1016/j.yexmp.2015.06.020

M3 - Article

C2 - 26123930

AN - SCOPUS:84937911840

VL - 99

SP - 229

EP - 235

JO - Experimental and Molecular Pathology

JF - Experimental and Molecular Pathology

SN - 0014-4800

IS - 2

ER -

Protective effect of ischemic preconditioning on the jejunal graft mucosa injury during cold preservation

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this