Protective effect of melatonin against the inflammatory response elicited by crude venom from isolated nematocysts of Pelagia noctiluca (Cnidaria, Scyphozoa)

Angela Marino, Rosanna Di Paola, Concetta Crisafulli, Emanuela Mazzon, Rossana Morabito, Irene Paterniti, Maria Galuppo, Tiziana Genovese, Giuseppina La Spada, Salvatore Cuzzocrea

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Melatonin (N-acetyl-5-methoxytryptamine) is an efficient free radical scavenger and antioxidant, both in vitro and in vivo. The role of melatonin as an immunomodulator is, in some cases, contradictory. In this study we have investigated the therapeutic efficacy of melatonin in rats subjected to Pelagia noctiluca crude venom (of the familia Pelaguiidae; and genus Pelagia) induced acute paw inflammation. In particular, injection of the venom into the paw of rats elicited an acute inflammatory response characterized by accumulation of fluid containing a large number of polymorphonuclear neutrophils in the paw and subsequent lipid peroxidation. Furthermore, the venom promoted an expression of iNOS, nitrotyrosine and the activation of the nuclear enzyme poly (ADP-ribose) polymerase as determined by immunohistochemical analysis of paw tissues. Administration of melatonin 30 min, 1 and 6 hr after the challenge with the venom, caused a significant reduction in all the parameters of inflammation measured. Thus, based on these findings we propose that melatonin may be useful a treatment of local acute inflammation induced by P. noctiluca crude venom.

Original languageEnglish
Pages (from-to)56-69
Number of pages14
JournalJournal of Pineal Research
Volume47
Issue number1
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Scyphozoa
Cnidarian Venoms
Cnidaria
Venoms
Melatonin
Inflammation
5-Methoxytryptamine
Free Radical Scavengers
Enzyme Activation
Poly(ADP-ribose) Polymerases
Immunologic Factors
Lipid Peroxidation
Neutrophils
Antioxidants
Injections
Therapeutics

Keywords

  • Inducible nitric oxide synthase
  • Inflammation
  • Melatonin
  • NF-κB
  • Nitric oxide
  • Oxidative stress
  • Pelagia noctiluca

ASJC Scopus subject areas

  • Endocrinology

Cite this

Protective effect of melatonin against the inflammatory response elicited by crude venom from isolated nematocysts of Pelagia noctiluca (Cnidaria, Scyphozoa). / Marino, Angela; Di Paola, Rosanna; Crisafulli, Concetta; Mazzon, Emanuela; Morabito, Rossana; Paterniti, Irene; Galuppo, Maria; Genovese, Tiziana; La Spada, Giuseppina; Cuzzocrea, Salvatore.

In: Journal of Pineal Research, Vol. 47, No. 1, 08.2009, p. 56-69.

Research output: Contribution to journalArticle

Marino, Angela ; Di Paola, Rosanna ; Crisafulli, Concetta ; Mazzon, Emanuela ; Morabito, Rossana ; Paterniti, Irene ; Galuppo, Maria ; Genovese, Tiziana ; La Spada, Giuseppina ; Cuzzocrea, Salvatore. / Protective effect of melatonin against the inflammatory response elicited by crude venom from isolated nematocysts of Pelagia noctiluca (Cnidaria, Scyphozoa). In: Journal of Pineal Research. 2009 ; Vol. 47, No. 1. pp. 56-69.
@article{69ced33ed1f0463097b146319be9cbb0,
title = "Protective effect of melatonin against the inflammatory response elicited by crude venom from isolated nematocysts of Pelagia noctiluca (Cnidaria, Scyphozoa)",
abstract = "Melatonin (N-acetyl-5-methoxytryptamine) is an efficient free radical scavenger and antioxidant, both in vitro and in vivo. The role of melatonin as an immunomodulator is, in some cases, contradictory. In this study we have investigated the therapeutic efficacy of melatonin in rats subjected to Pelagia noctiluca crude venom (of the familia Pelaguiidae; and genus Pelagia) induced acute paw inflammation. In particular, injection of the venom into the paw of rats elicited an acute inflammatory response characterized by accumulation of fluid containing a large number of polymorphonuclear neutrophils in the paw and subsequent lipid peroxidation. Furthermore, the venom promoted an expression of iNOS, nitrotyrosine and the activation of the nuclear enzyme poly (ADP-ribose) polymerase as determined by immunohistochemical analysis of paw tissues. Administration of melatonin 30 min, 1 and 6 hr after the challenge with the venom, caused a significant reduction in all the parameters of inflammation measured. Thus, based on these findings we propose that melatonin may be useful a treatment of local acute inflammation induced by P. noctiluca crude venom.",
keywords = "Inducible nitric oxide synthase, Inflammation, Melatonin, NF-κB, Nitric oxide, Oxidative stress, Pelagia noctiluca",
author = "Angela Marino and {Di Paola}, Rosanna and Concetta Crisafulli and Emanuela Mazzon and Rossana Morabito and Irene Paterniti and Maria Galuppo and Tiziana Genovese and {La Spada}, Giuseppina and Salvatore Cuzzocrea",
year = "2009",
month = "8",
doi = "10.1111/j.1600-079X.2009.00688.x",
language = "English",
volume = "47",
pages = "56--69",
journal = "Journal of Pineal Research",
issn = "0742-3098",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Protective effect of melatonin against the inflammatory response elicited by crude venom from isolated nematocysts of Pelagia noctiluca (Cnidaria, Scyphozoa)

AU - Marino, Angela

AU - Di Paola, Rosanna

AU - Crisafulli, Concetta

AU - Mazzon, Emanuela

AU - Morabito, Rossana

AU - Paterniti, Irene

AU - Galuppo, Maria

AU - Genovese, Tiziana

AU - La Spada, Giuseppina

AU - Cuzzocrea, Salvatore

PY - 2009/8

Y1 - 2009/8

N2 - Melatonin (N-acetyl-5-methoxytryptamine) is an efficient free radical scavenger and antioxidant, both in vitro and in vivo. The role of melatonin as an immunomodulator is, in some cases, contradictory. In this study we have investigated the therapeutic efficacy of melatonin in rats subjected to Pelagia noctiluca crude venom (of the familia Pelaguiidae; and genus Pelagia) induced acute paw inflammation. In particular, injection of the venom into the paw of rats elicited an acute inflammatory response characterized by accumulation of fluid containing a large number of polymorphonuclear neutrophils in the paw and subsequent lipid peroxidation. Furthermore, the venom promoted an expression of iNOS, nitrotyrosine and the activation of the nuclear enzyme poly (ADP-ribose) polymerase as determined by immunohistochemical analysis of paw tissues. Administration of melatonin 30 min, 1 and 6 hr after the challenge with the venom, caused a significant reduction in all the parameters of inflammation measured. Thus, based on these findings we propose that melatonin may be useful a treatment of local acute inflammation induced by P. noctiluca crude venom.

AB - Melatonin (N-acetyl-5-methoxytryptamine) is an efficient free radical scavenger and antioxidant, both in vitro and in vivo. The role of melatonin as an immunomodulator is, in some cases, contradictory. In this study we have investigated the therapeutic efficacy of melatonin in rats subjected to Pelagia noctiluca crude venom (of the familia Pelaguiidae; and genus Pelagia) induced acute paw inflammation. In particular, injection of the venom into the paw of rats elicited an acute inflammatory response characterized by accumulation of fluid containing a large number of polymorphonuclear neutrophils in the paw and subsequent lipid peroxidation. Furthermore, the venom promoted an expression of iNOS, nitrotyrosine and the activation of the nuclear enzyme poly (ADP-ribose) polymerase as determined by immunohistochemical analysis of paw tissues. Administration of melatonin 30 min, 1 and 6 hr after the challenge with the venom, caused a significant reduction in all the parameters of inflammation measured. Thus, based on these findings we propose that melatonin may be useful a treatment of local acute inflammation induced by P. noctiluca crude venom.

KW - Inducible nitric oxide synthase

KW - Inflammation

KW - Melatonin

KW - NF-κB

KW - Nitric oxide

KW - Oxidative stress

KW - Pelagia noctiluca

UR - http://www.scopus.com/inward/record.url?scp=67649939036&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649939036&partnerID=8YFLogxK

U2 - 10.1111/j.1600-079X.2009.00688.x

DO - 10.1111/j.1600-079X.2009.00688.x

M3 - Article

VL - 47

SP - 56

EP - 69

JO - Journal of Pineal Research

JF - Journal of Pineal Research

SN - 0742-3098

IS - 1

ER -