Protective effect of methylprednisolone and of intermittent hepatic pedicle clamping during liver vascular inflow occlusion in the rat

Antonio C. Chiappa, Masatoshi Makuuchi, Andrew P. Zbar, Francesca Biella, Aldo Vezzoni, Guido Torzilli, Bruno Andreoni

Research output: Contribution to journalArticle

Abstract

Background/Aims: One of the main causes of postoperative morbidity and mortality following major hepatic resection is hepatic ischemia deliberately designed to reduce intraoperative hemorrhage. This study assessed the effects of intermittent or continuous hepatic ischemia and reperfusion with or without methylprednisolone pretreatment in the rat. Methodology: One hundred and eighty rats were divided into 3 groups undergoing hepatic ischemia of 60, 90, and 120 minutes total duration. Each group of rats were subdivided to receive either a continuous Pringle maneuver, or 30 min or 15 min of intermittent liver pedicle clamping. Ten minutes before ischemia induction, 10 rats from each group were pretreated with intravenous 3mg/100g bw methylprednisolone. Results: With continuous hepatic pedicle clamping the rat survival rates inversely correlated with the duration of ischemia (survival: 70%, 40%, and 20% with ischemia of 60, 90, and 120 min). Survival rates at 15-min and 30-min intermittent ischemia groups were significantly higher than in the continuous clamping group (p

Original languageEnglish
Pages (from-to)1439-1444
Number of pages6
JournalHepato-Gastroenterology
Volume51
Issue number59
Publication statusPublished - Sep 2004

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Keywords

  • Energy charge
  • Hepatic vascular occlusion
  • Ischemic injury of the liver
  • Liver tissue adenosine-5′-triphosphate

ASJC Scopus subject areas

  • Gastroenterology

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