Protective effect of nedocromil sodium on the interleukin-1-induced production of interleukin-8 in human bronchial epithelial cells

Enza Vittori, Fanny Sciacca, Francesco Colotta, Alberto Mantovani, Sabrina Mattoli

Research output: Contribution to journalArticlepeer-review

Abstract

Cellular constituents of the bronchial mucosa may participate in the recruitment of polymorphonuclear leukocytes to the inflammed airways through the generation of the neutrophil chemotactic peptide, interleukin-8 (IL-8). One important aspect of this interaction could be represented by the ability of pulmonary monokines, particularly IL-1, to induce gene expression of IL-8 in bronchial epithelial cells. In this study, we have evaluated the constitutive and IL-1-induced production of IL-8 by cultured human bronchial epithelial cells and examined the ability of the anti-inflammatory drug, nedocromil sodium, to inhibit the cytokine synthesis and release. Northern blot analysis demonstrated that IL-1β-treated human bronchial epithelial cells expressed appreciable levels of IL-8 mRNA during a period of 8 hours. This finding was associated with an increased release of immunoreactive IL-8 to the culture media, as assessed by specific ELISA. The bronchial epithelial cell-derived IL-8 demonstrated specific biologic activity, since the supernatants of stimulated bronchial epithelial cells possessed neutrophil chemotactic activity that could be inhibited by an antibody against human IL-8. Nedocromil sodium reduced the IL-1-induced release of IL-8 in a dose-dependent manner, but concentrations of the compounds, up to 10-5 mol/L, did not affect the constitutive production of this cytokine.

Original languageEnglish
Pages (from-to)76-84
Number of pages9
JournalJournal of Allergy and Clinical Immunology
Volume90
Issue number1
DOIs
Publication statusPublished - 1992

Keywords

  • airway epithelium
  • inflammation
  • Interleukin-8

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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