Protective effects of n-acetylcysteine on lung injury and red blood cell modification induced by carrageenan in the rat

Salvatore Cuzzocrea, Emanuela Mazzon, Laura Dugo, Ivana Serraino, Antonio Ciccolo, Tommaso Centorrino, Angela De Sarro, Achille P. Caputi

Research output: Contribution to journalArticlepeer-review

Abstract

Oxidative stress has been suggested as a potential mechanism in the pathogenesis of lung inflammation. The pharmacological profile of n-acetylcysteine (NAC), a free radical scavenger, was evaluated in an experimental model of lung injury (carrageenan-induced pleurisy). Injection of carrageenan into the pleural cavity of rats elicited an acute inflammatory response characterized by fluid accumulation in the pleural cavity that contained many neutrophils (PMNs), an infiltration of PMNs in lung tissues and subsequent lipid peroxidation, and increased production of nitrite/nitrate, tumor necrosis factor α, and interleukin 1 β. All parameters of inflammation were attenuated by NAC treatment. Furthermore, carrageenan induced an up-regulation of the adhesion molecules ICAM-1 and P-selectin, as well as nitrotyrosine and poly (ADP-ribose) synthetase (PARS), as determined by immunohistochemical analysis of lung tissues. The degree of staining for the ICAM-1, P-selectin, nitrotyrosine, and PARS was reduced by NAC. In vivo NAC treatment significantly reduced peroxynitrite formation as measured by the oxidation of the fluorescent dihydrorhodamine-123, prevented the appearance of DNA damage, an decrease in mitochondrial respiration, and partially restored the cellular level of NAD+ in ex vivo macrophages harvested from the pleural cavity of rats subjected to carrageenan-induced pleurisy. A significant alteration in the morphology of red blood cells was observed 24 h after carrageenan administration. NAC treatment has the ability to significantly diminish the red blood cell alteration. Our results clearly demonstrate that NAC treatment exerts a protective effect and clearly indicate that NAC offers a novel therapeutic approach for the management of lung injury where radicals have been postulated to play a role. - Cuzzocrea, S., Mazzon, E., Dugo, L., Serraino, I., Ciccolo, A., Centorrino, T., De Sarro, A., Caputi, A. P. Protective effects of n-acetylcysteine on lung injury and red blood cell modification induced by carrageenan in the rat.

Original languageEnglish
Pages (from-to)1187-1200
Number of pages14
JournalFASEB Journal
Volume15
Issue number7
DOIs
Publication statusPublished - 2001

Keywords

  • Inflammation
  • N-acetylcysteine
  • Nitric oxide
  • Peroxynitrite
  • Superoxide

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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