Protective role of hydrogen peroxide in oxygen-deprived dopaminergic neurones of the rat substantia nigra

Raffaella Geracitano, Alessandro Tozzi, Nicola Berretta, Fulvio Florenzano, Ezia Guatteo, Maria Teresa Viscomi, Barbara Chiolo, Marco Molinari, Giorgio Bernardi, Nicola B. Mercuri

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Hydrogen peroxide (H2O2) is a reactive oxygen species, responsible for cytotoxic damage through the formation of hydroxyl radicals. Dopamine (DA) neurones of the substantia nigra pars compacta (SNc) are highly sensitive to metabolic stress, and they typically respond to energy deprivation with membrane hyperpolarization, mainly through opening of ATP-dependent K+ channels. Accordingly, H2O2 (3 mM) induced a tolbutamide-sensitive outward current in DA neurones. Conversely, in a hypoxic medium, H2O2 reverted membrane hyperpolarization, which is associated with oxygen deprivation in DA neurones, restored their action potential firing, and reduced the hypoxia-mediated outward current in a concentration-dependent manner, between 0.1 and 3 mM (IC50 0.6 ± 0.1 mM). Notably, H2O2 did not counteract membrane hyperpolarization associated with hypoglycaemia, moreover, when catalase was inhibited with 3-amino-1,2,4-triazole (3-AT; 30 mM), H2O2 did not reduce hypoxia-mediated outward current. The counteracting action of H2O2 on hypoxia-mediated effects was further confirmed. by single-unit extracellular recordings of presumed DA neurones in acute midbrain slices preparations, using a planar multi-electrode array device. Whilst a prolonged period of hypoxia (40 min) caused firing suppression, which did not recover after perfusion in normoxic conditions, the presence of H2O2 (3 mM) during this prolonged hypoxic period rescued most of the neurones from irreversible firing inhibition. Accordingly, morphological studies showed that H2O2 counteracts the cytochrome c release provoked by prolonged hypoxic treatment. Taken together, our data suggest that H2O2 prevents the metabolic stress of DA neurones induced by hypoxia by serving as a supplementary source of molecular oxygen, through its degradation by catalase.

Original languageEnglish
Pages (from-to)97-110
Number of pages14
JournalJournal of Physiology
Volume568
Issue number1
DOIs
Publication statusPublished - Oct 1 2005

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Dopaminergic Neurons
Substantia Nigra
Hydrogen Peroxide
Oxygen
Physiological Stress
Catalase
Membranes
Amitrole
Tolbutamide
Mesencephalon
Cytochromes c
Hypoglycemia
Hydroxyl Radical
Action Potentials
Inhibitory Concentration 50
Reactive Oxygen Species
Electrodes
Perfusion
Adenosine Triphosphate
Hypoxia

ASJC Scopus subject areas

  • Physiology

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Protective role of hydrogen peroxide in oxygen-deprived dopaminergic neurones of the rat substantia nigra. / Geracitano, Raffaella; Tozzi, Alessandro; Berretta, Nicola; Florenzano, Fulvio; Guatteo, Ezia; Viscomi, Maria Teresa; Chiolo, Barbara; Molinari, Marco; Bernardi, Giorgio; Mercuri, Nicola B.

In: Journal of Physiology, Vol. 568, No. 1, 01.10.2005, p. 97-110.

Research output: Contribution to journalArticle

Geracitano, Raffaella ; Tozzi, Alessandro ; Berretta, Nicola ; Florenzano, Fulvio ; Guatteo, Ezia ; Viscomi, Maria Teresa ; Chiolo, Barbara ; Molinari, Marco ; Bernardi, Giorgio ; Mercuri, Nicola B. / Protective role of hydrogen peroxide in oxygen-deprived dopaminergic neurones of the rat substantia nigra. In: Journal of Physiology. 2005 ; Vol. 568, No. 1. pp. 97-110.
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AU - Florenzano, Fulvio

AU - Guatteo, Ezia

AU - Viscomi, Maria Teresa

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AU - Bernardi, Giorgio

AU - Mercuri, Nicola B.

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