Protein and Molecular Characterization of a Clinically Compliant Amniotic Fluid Stem Cell-Derived Extracellular Vesicle Fraction Capable of Accelerating Muscle Regeneration Through Enhancement of Angiogenesis

Ben Mellows, Robert Mitchell, Manuela Antonioli, Oliver Kretz, David Chambers, Marie Theres Zeuner, Bernd Denecke, Luca Musante, Durrgah L. Ramachandra, Florence Debacq-Chainiaux, Harry Holthofer, Barbara Joch, Steve Ray, Darius Widera, Anna L. David, Tobias B. Huber, Joern Dengjel, Paolo De Coppi, Ketan Patel

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The secretome of human amniotic fluid stem cells (AFSCs) has great potential as a therapeutic agent in regenerative medicine. However, it must be produced in a clinically compliant manner before it can be used in humans. In this study, we developed a means of producing a biologically active secretome from AFSCs that is free of all exogenous molecules. We demonstrate that the full secretome is capable of promoting stem cell proliferation, migration, and protection of cells against senescence. Furthermore, it has significant anti-inflammatory properties. Most importantly, we show that it promotes tissue regeneration in a model of muscle damage. We then demonstrate that the secretome contains extracellular vesicles (EVs) that harbor much, but not all, of the biological activity of the whole secretome. Proteomic characterization of the EV and free secretome fraction shows the presence of numerous molecules specific to each fraction that could be key regulators of tissue regeneration. Intriguingly, we show that the EVs only contain miRNA and not mRNA. This suggests that tissue regeneration in the host is mediated by the action of EVs modifying existing, rather than imposing new, signaling pathways. The EVs harbor significant anti-inflammatory activity as well as promote angiogenesis, the latter may be the mechanistic explanation for their ability to promote muscle regeneration after cardiotoxin injury.

Original languageEnglish
Pages (from-to)1316-1333
Number of pages18
JournalStem Cells and Development
Volume26
Issue number18
DOIs
Publication statusPublished - Sep 15 2017

Fingerprint

Amniotic Fluid
Regeneration
Stem Cells
Muscles
Proteins
Anti-Inflammatory Agents
Cardiotoxins
Regenerative Medicine
Cytoprotection
Cell Aging
MicroRNAs
Proteomics
Cell Movement
Cell Proliferation
Extracellular Vesicles
Messenger RNA
Wounds and Injuries

Keywords

  • miRNA
  • muscle
  • regeneration
  • secretome

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

Cite this

Protein and Molecular Characterization of a Clinically Compliant Amniotic Fluid Stem Cell-Derived Extracellular Vesicle Fraction Capable of Accelerating Muscle Regeneration Through Enhancement of Angiogenesis. / Mellows, Ben; Mitchell, Robert; Antonioli, Manuela; Kretz, Oliver; Chambers, David; Zeuner, Marie Theres; Denecke, Bernd; Musante, Luca; Ramachandra, Durrgah L.; Debacq-Chainiaux, Florence; Holthofer, Harry; Joch, Barbara; Ray, Steve; Widera, Darius; David, Anna L.; Huber, Tobias B.; Dengjel, Joern; De Coppi, Paolo; Patel, Ketan.

In: Stem Cells and Development, Vol. 26, No. 18, 15.09.2017, p. 1316-1333.

Research output: Contribution to journalArticle

Mellows, B, Mitchell, R, Antonioli, M, Kretz, O, Chambers, D, Zeuner, MT, Denecke, B, Musante, L, Ramachandra, DL, Debacq-Chainiaux, F, Holthofer, H, Joch, B, Ray, S, Widera, D, David, AL, Huber, TB, Dengjel, J, De Coppi, P & Patel, K 2017, 'Protein and Molecular Characterization of a Clinically Compliant Amniotic Fluid Stem Cell-Derived Extracellular Vesicle Fraction Capable of Accelerating Muscle Regeneration Through Enhancement of Angiogenesis', Stem Cells and Development, vol. 26, no. 18, pp. 1316-1333. https://doi.org/10.1089/scd.2017.0089
Mellows, Ben ; Mitchell, Robert ; Antonioli, Manuela ; Kretz, Oliver ; Chambers, David ; Zeuner, Marie Theres ; Denecke, Bernd ; Musante, Luca ; Ramachandra, Durrgah L. ; Debacq-Chainiaux, Florence ; Holthofer, Harry ; Joch, Barbara ; Ray, Steve ; Widera, Darius ; David, Anna L. ; Huber, Tobias B. ; Dengjel, Joern ; De Coppi, Paolo ; Patel, Ketan. / Protein and Molecular Characterization of a Clinically Compliant Amniotic Fluid Stem Cell-Derived Extracellular Vesicle Fraction Capable of Accelerating Muscle Regeneration Through Enhancement of Angiogenesis. In: Stem Cells and Development. 2017 ; Vol. 26, No. 18. pp. 1316-1333.
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