Protein corona: Implications for nanoparticle interactions with pulmonary cells

Nagarjun V. Konduru, Ramon M. Molina, Archana Swami, Flavia Damiani, Georgios Pyrgiotakis, Paulo Lin, Patrizia Andreozzi, Thomas C. Donaghey, Philip Demokritou, Silke Krol, Wolfgang Kreyling, Joseph D. Brain

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: We previously showed that cerium oxide (CeO2), barium sulfate (BaSO4) and zinc oxide (ZnO) nanoparticles (NPs) exhibited different lung toxicity and pulmonary clearance in rats. We hypothesize that these NPs acquire coronas with different protein compositions that may influence their clearance from the lungs. Methods: CeO2, silica-coated CeO2, BaSO4, and ZnO NPs were incubated in rat lung lining fluid in vitro. Then, gel electrophoresis followed by quantitative mass spectrometry was used to characterize the adsorbed proteins stripped from these NPs. We also measured uptake of instilled NPs by alveolar macrophages (AMs) in rat lungs using electron microscopy. Finally, we tested whether coating of gold NPs with albumin would alter their lung clearance in rats. Results: We found that the amounts of nine proteins in the coronas formed on the four NPs varied significantly. The amounts of albumin, transferrin and α-1 antitrypsin were greater in the coronas of BaSO4 and ZnO than that of the two CeO2 NPs. The uptake of BaSO4 in AMs was less than CeO2 and silica-coated CeO2 NPs. No identifiable ZnO NPs were observed in AMs. Gold NPs coated with albumin or citrate instilled into the lungs of rats acquired the similar protein coronas and were cleared from the lungs to the same extent. Conclusions: We show that different NPs variably adsorb proteins from the lung lining fluid. The amount of albumin in the NP corona varies as does NP uptake by AMs. However, albumin coating does not affect the translocation of gold NPs across the air-blood barrier. A more extensive database of corona composition of a diverse NP library will develop a platform to help predict the effects and biokinetics of inhaled NPs.

Original languageEnglish
Article number42
JournalParticle and Fibre Toxicology
Volume14
Issue number1
DOIs
Publication statusPublished - Oct 30 2017

Fingerprint

Nanoparticles
Lung
Zinc Oxide
Alveolar Macrophages
Rats
Albumins
Gold
Protein Corona
Linings
Silicon Dioxide
Proteins
Blood-Air Barrier
Barium Sulfate
Coatings
Fluids
Transferrin
Electrophoresis
Chemical analysis
Citric Acid
Electron microscopy

Keywords

  • Biokinetics
  • Engineered nanoparticles
  • Lung macrophage
  • Nanotoxicity
  • Protein corona

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Konduru, N. V., Molina, R. M., Swami, A., Damiani, F., Pyrgiotakis, G., Lin, P., ... Brain, J. D. (2017). Protein corona: Implications for nanoparticle interactions with pulmonary cells. Particle and Fibre Toxicology, 14(1), [42]. https://doi.org/10.1186/s12989-017-0223-3

Protein corona : Implications for nanoparticle interactions with pulmonary cells. / Konduru, Nagarjun V.; Molina, Ramon M.; Swami, Archana; Damiani, Flavia; Pyrgiotakis, Georgios; Lin, Paulo; Andreozzi, Patrizia; Donaghey, Thomas C.; Demokritou, Philip; Krol, Silke; Kreyling, Wolfgang; Brain, Joseph D.

In: Particle and Fibre Toxicology, Vol. 14, No. 1, 42, 30.10.2017.

Research output: Contribution to journalArticle

Konduru, NV, Molina, RM, Swami, A, Damiani, F, Pyrgiotakis, G, Lin, P, Andreozzi, P, Donaghey, TC, Demokritou, P, Krol, S, Kreyling, W & Brain, JD 2017, 'Protein corona: Implications for nanoparticle interactions with pulmonary cells', Particle and Fibre Toxicology, vol. 14, no. 1, 42. https://doi.org/10.1186/s12989-017-0223-3
Konduru, Nagarjun V. ; Molina, Ramon M. ; Swami, Archana ; Damiani, Flavia ; Pyrgiotakis, Georgios ; Lin, Paulo ; Andreozzi, Patrizia ; Donaghey, Thomas C. ; Demokritou, Philip ; Krol, Silke ; Kreyling, Wolfgang ; Brain, Joseph D. / Protein corona : Implications for nanoparticle interactions with pulmonary cells. In: Particle and Fibre Toxicology. 2017 ; Vol. 14, No. 1.
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AU - Swami, Archana

AU - Damiani, Flavia

AU - Pyrgiotakis, Georgios

AU - Lin, Paulo

AU - Andreozzi, Patrizia

AU - Donaghey, Thomas C.

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AU - Kreyling, Wolfgang

AU - Brain, Joseph D.

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N2 - Background: We previously showed that cerium oxide (CeO2), barium sulfate (BaSO4) and zinc oxide (ZnO) nanoparticles (NPs) exhibited different lung toxicity and pulmonary clearance in rats. We hypothesize that these NPs acquire coronas with different protein compositions that may influence their clearance from the lungs. Methods: CeO2, silica-coated CeO2, BaSO4, and ZnO NPs were incubated in rat lung lining fluid in vitro. Then, gel electrophoresis followed by quantitative mass spectrometry was used to characterize the adsorbed proteins stripped from these NPs. We also measured uptake of instilled NPs by alveolar macrophages (AMs) in rat lungs using electron microscopy. Finally, we tested whether coating of gold NPs with albumin would alter their lung clearance in rats. Results: We found that the amounts of nine proteins in the coronas formed on the four NPs varied significantly. The amounts of albumin, transferrin and α-1 antitrypsin were greater in the coronas of BaSO4 and ZnO than that of the two CeO2 NPs. The uptake of BaSO4 in AMs was less than CeO2 and silica-coated CeO2 NPs. No identifiable ZnO NPs were observed in AMs. Gold NPs coated with albumin or citrate instilled into the lungs of rats acquired the similar protein coronas and were cleared from the lungs to the same extent. Conclusions: We show that different NPs variably adsorb proteins from the lung lining fluid. The amount of albumin in the NP corona varies as does NP uptake by AMs. However, albumin coating does not affect the translocation of gold NPs across the air-blood barrier. A more extensive database of corona composition of a diverse NP library will develop a platform to help predict the effects and biokinetics of inhaled NPs.

AB - Background: We previously showed that cerium oxide (CeO2), barium sulfate (BaSO4) and zinc oxide (ZnO) nanoparticles (NPs) exhibited different lung toxicity and pulmonary clearance in rats. We hypothesize that these NPs acquire coronas with different protein compositions that may influence their clearance from the lungs. Methods: CeO2, silica-coated CeO2, BaSO4, and ZnO NPs were incubated in rat lung lining fluid in vitro. Then, gel electrophoresis followed by quantitative mass spectrometry was used to characterize the adsorbed proteins stripped from these NPs. We also measured uptake of instilled NPs by alveolar macrophages (AMs) in rat lungs using electron microscopy. Finally, we tested whether coating of gold NPs with albumin would alter their lung clearance in rats. Results: We found that the amounts of nine proteins in the coronas formed on the four NPs varied significantly. The amounts of albumin, transferrin and α-1 antitrypsin were greater in the coronas of BaSO4 and ZnO than that of the two CeO2 NPs. The uptake of BaSO4 in AMs was less than CeO2 and silica-coated CeO2 NPs. No identifiable ZnO NPs were observed in AMs. Gold NPs coated with albumin or citrate instilled into the lungs of rats acquired the similar protein coronas and were cleared from the lungs to the same extent. Conclusions: We show that different NPs variably adsorb proteins from the lung lining fluid. The amount of albumin in the NP corona varies as does NP uptake by AMs. However, albumin coating does not affect the translocation of gold NPs across the air-blood barrier. A more extensive database of corona composition of a diverse NP library will develop a platform to help predict the effects and biokinetics of inhaled NPs.

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