A screening of the most relevant histochemical parameters for the demonstration of polysaccharides, proteins, ribonucleic acid, enzymes related to glycogen metabolism (total phosphorylase, UDPG-glucose-glycogen transferase) and enzymes related to tissue energy metabolism (oxidoreductase, diaphorase, acid and alkaline phosphatases, ATPase) was carried out in order to point out differences between normal placentas and protein-deprived ones (8% proteins for 15 days) at the 19th day. Histochemically, the decreased placental efficiency (as shown by weight drop of malnourished placentas) was revealed only by a reduction of the glycogen level and by a different distribution of the enzymes related to its metabolism. A microdensitometric evaluation of DNA content (Feulgen positive material) in relation to nuclear area, and of total nuclear proteins (Naphthol yellow positive material) of trophoblast giant cells showed that, though ploidy classes were identical, nuclear areas and protein contents exhibited changes which varied according to the degree of ploidy.
|Translated title of the contribution||Protein-depriving and placental efficiency: Histochemical findings|
|Number of pages||16|
|Journal||Annali di Ostetricia Ginecologia Medicina Perinatale|
|Publication status||Published - 1978|
ASJC Scopus subject areas
- Obstetrics and Gynaecology