Protein kinase C isoenzymes in human neuroblasts involvement of PKCε in cell differentiation

M. Ponzoni, E. Lucarelli, M. V. Corrias, P. Cornaglia-Ferraris

Research output: Contribution to journalArticle

Abstract

Although neuronal cells are a major target of phorbol ester action, the activity of the various protein kinase C (PKC) isoenzymes have not been studied in detail in human neuroblasts. Differentiation of the LAN-5 human neuroblastoma cell line by interferon-γ (IFN-γ) is accompanied by a twofold increase in PKC activity. Since PKC is a multigene family, we investigated which isoforms were expressed in control and differentiated cells, and which of these isoenzymes is involved in neuronal differentiation. We found that: (1) PKC activity is higher in differentiated than in undifferentiated cells; (2) RT-PCR analysis showed the expression of mRNA for PKCα, -γ, -δ -ε and-ζ and the absence of mRNA for β in untreated LAN-5 cells; (3) Western blot evaluation with PKC isoform-specific antibodies showed the same pattern of PKC expression in non-differentiated cells; (4) Expression of PKCε mRNA was significantly enhanced by IFN-γ-induced differentiation, while the other isoforms were not affected; (5) Differentiation of LAN-5 cells with IFN-γ or retinoic acid induced overexpression of the PKCε protein, while inhibition of cell proliferation by fetal calf serum starvation was without effect. These findings suggest that expression of PKCε isoform is tightly coupled with neuronal differentiation and may play a role in the maintenance of the differentiated state.

Original languageEnglish
Pages (from-to)120-124
Number of pages5
JournalFEBS Letters
Volume322
Issue number2
DOIs
Publication statusPublished - May 10 1993

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Protein Kinase C
Isoenzymes
Cell Differentiation
Local Area Networks
Protein Isoforms
Local area networks
Interferons
Messenger RNA
Cell proliferation
Phorbol Esters
Multigene Family
Starvation
Tretinoin
Neuroblastoma
Western Blotting
Cells
Maintenance
Cell Proliferation
Cell Line
Polymerase Chain Reaction

Keywords

  • Cell differentiation
  • Isoform
  • Neuroblastoma
  • Protein kinase C

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Protein kinase C isoenzymes in human neuroblasts involvement of PKCε in cell differentiation. / Ponzoni, M.; Lucarelli, E.; Corrias, M. V.; Cornaglia-Ferraris, P.

In: FEBS Letters, Vol. 322, No. 2, 10.05.1993, p. 120-124.

Research output: Contribution to journalArticle

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