Protein kinase C isoforms undergo quantitative variations during rat spermatogenesis and are selectively retained at specific spermatozoon sites

Nicoletta Zini, Alessandro Matteucci, Patrizia Sabatelli, Aurelio Valmori, Elisabetta Caramelli, Nadir M. Maraldi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Signal transduction elements, including protein kinase C, have been identified in mammalian spermatozoa. In order to evaluate the pattern of expression and the subcellular localization of nine different protein kinase C isoforms in the course of spermatogenesis, we utilized quantitative electron microscopy immunocytochemistry on thin sections of rat seminiferous tubules. The results indicate a progressive reduction of the protein kinase C isoforms present in the early stages of spermatogenesis, so that in late spermatids none of them is present in the nucleus, while the isoforms α, γ and β(II) are specifically retained in the acrosome, the isoforms β(I) and ζ in the neck, and the isoform ε in the tail. These isoforms, except for β(II), are maintained at the same sites in spermatozoa. Western blotting analysis indicates the presence of α and γ isoforms in the head subfraction, and of β(I), ζ and ε isoforms in the tail subfraction of spermatozoa. These findings suggest that specific protein kinase C isoforms may be functionally involved in some events of spermatozoa differentiation and, eventually, in the fertilization process.

Original languageEnglish
Pages (from-to)142-150
Number of pages9
JournalEuropean Journal of Cell Biology
Volume72
Issue number2
Publication statusPublished - 1997

Fingerprint

Spermatogenesis
Protein Kinase C
Spermatozoa
Protein Isoforms
Tail
Acrosome
Seminiferous Tubules
Spermatids
Fertilization
Signal Transduction
Electron Microscopy
Neck
Western Blotting
Immunohistochemistry
Head

Keywords

  • Immunocytochemistry
  • Protein kinase C
  • Signal transduction
  • Spermatogenesis
  • Spermatozoa

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Protein kinase C isoforms undergo quantitative variations during rat spermatogenesis and are selectively retained at specific spermatozoon sites. / Zini, Nicoletta; Matteucci, Alessandro; Sabatelli, Patrizia; Valmori, Aurelio; Caramelli, Elisabetta; Maraldi, Nadir M.

In: European Journal of Cell Biology, Vol. 72, No. 2, 1997, p. 142-150.

Research output: Contribution to journalArticle

Zini, Nicoletta ; Matteucci, Alessandro ; Sabatelli, Patrizia ; Valmori, Aurelio ; Caramelli, Elisabetta ; Maraldi, Nadir M. / Protein kinase C isoforms undergo quantitative variations during rat spermatogenesis and are selectively retained at specific spermatozoon sites. In: European Journal of Cell Biology. 1997 ; Vol. 72, No. 2. pp. 142-150.
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AU - Caramelli, Elisabetta

AU - Maraldi, Nadir M.

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