Protein kinase C mRNA levels and activity in reconstituted normal human epidermis: Relationships to cell differentiation

Roberto Gherzi, Bianca Sparatore, Mauro Patrone, Andrea Sciutto, Paola Briata

Research output: Contribution to journalArticlepeer-review

Abstract

Although keratinocytes are a major target of phorbol ester actions, the activity and the expression of the eight cloned protein kinase C (PKC) isoenzymes have not been studied in detail in human epidermis. Starting from normal human keratinocytes, we reconstituted in culture a mutilayered epithelial tissue which presents many hystological, biochemical, and molecular features of the authentic epidermis and we used it as a model to investigate the PKC activity and mRNA levels. We found that i) PKC activity is higher in differentiated than in non-differentiated cells; ii) the mRNA levels of PKCδ and -η L, while are differently affected by spontaneous keratinocyte differentiation, are down-regulated during phorbol esters-induced cell differentiation. Our findings could represent a basis to investigate the involvement of PKC isoforms in the keratinocyte differentiation process.

Original languageEnglish
Pages (from-to)283-291
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume184
Issue number1
DOIs
Publication statusPublished - Apr 15 1992

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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