Protein kinase CK2 holoenzyme promotes start-specific transcription in saccharomyces cerevisiae

Farida Tripodi, Raffaele Nicastro, Sara Busnelli, Claudia Cirulli, Elisa Maffioli, Gabriella Tedeschi, Lilia Alberghina, Paola Coccetti

Research output: Contribution to journalArticle

Abstract

In Saccharomyces cerevisiae, the entrance into S phase requires the activation of a specific burst of transcription, which depends on SBF (SCB binding factor, Swi4/Swi6) and MBF (MCB binding factor, Mbp1/Swi6) complexes. CK2 is a pleiotropic kinase involved in several cellular processes, including the regulation of the cell cycle. CK2 is composed of two catalytic subunits (α and α') and two regulatory subunits (β and β'), both of which are required to form the active holoenzyme. Here we investigate the function of the CK2 holoenzyme in Start-specific transcription. The ckb1Δ ckb2Δ mutant strain, bearing deletions of both genes encoding CK2 regulatory subunits, shows a delay of S-phase entrance due to a severe reduction of the expression of SBF- and MBF-dependent genes. This transcriptional defect is caused by an impaired recruitment of Swi6 and Swi4 to G1 gene promoters. Moreover, CK2 α and β' subunits interact with RNA polymerase II, whose binding to G1 promoters is positively regulated by the CK2 holoenzyme. Collectively, these findings suggest a novel role for the CK2 holoenzyme in the activation of G1 transcription.

Original languageEnglish
Pages (from-to)1271-1280
Number of pages10
JournalEukaryotic Cell
Volume12
Issue number9
DOIs
Publication statusPublished - Sep 2013

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Casein Kinase II
Holoenzymes
Saccharomyces cerevisiae
S Phase
RNA Polymerase II
Gene Deletion
Transcriptional Activation
Genes
Catalytic Domain
Cell Cycle
Phosphotransferases

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Tripodi, F., Nicastro, R., Busnelli, S., Cirulli, C., Maffioli, E., Tedeschi, G., ... Coccetti, P. (2013). Protein kinase CK2 holoenzyme promotes start-specific transcription in saccharomyces cerevisiae. Eukaryotic Cell, 12(9), 1271-1280. https://doi.org/10.1128/EC.00117-13

Protein kinase CK2 holoenzyme promotes start-specific transcription in saccharomyces cerevisiae. / Tripodi, Farida; Nicastro, Raffaele; Busnelli, Sara; Cirulli, Claudia; Maffioli, Elisa; Tedeschi, Gabriella; Alberghina, Lilia; Coccetti, Paola.

In: Eukaryotic Cell, Vol. 12, No. 9, 09.2013, p. 1271-1280.

Research output: Contribution to journalArticle

Tripodi, F, Nicastro, R, Busnelli, S, Cirulli, C, Maffioli, E, Tedeschi, G, Alberghina, L & Coccetti, P 2013, 'Protein kinase CK2 holoenzyme promotes start-specific transcription in saccharomyces cerevisiae', Eukaryotic Cell, vol. 12, no. 9, pp. 1271-1280. https://doi.org/10.1128/EC.00117-13
Tripodi, Farida ; Nicastro, Raffaele ; Busnelli, Sara ; Cirulli, Claudia ; Maffioli, Elisa ; Tedeschi, Gabriella ; Alberghina, Lilia ; Coccetti, Paola. / Protein kinase CK2 holoenzyme promotes start-specific transcription in saccharomyces cerevisiae. In: Eukaryotic Cell. 2013 ; Vol. 12, No. 9. pp. 1271-1280.
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