Protein-Metal Interactions Probed by SERS

Lysozyme on Nanostructured Gold Surface

N. R. Agarwal, M. Tommasini, E. Ciusani, A. Lucotti, S. Trusso, P. M. Ossi

Research output: Contribution to journalArticle

Abstract

Surface-enhanced Raman scattering is a well-established technique for molecular detection at low concentration, which is becoming increasingly popular in the field of biotechnology and health sciences. Since the process is understood in depth, the technique is becoming reliable. In this contribution, we consider another aspect of SERS besides molecular detection, focusing on the binding mechanisms of a complex system such as a protein to the noble metal substrates required by the technique itself. We also show that using a solid nanostructured substrate produced by controlled pulsed laser deposition SERS enables label-free detection of a protein. This is checked on lysozyme as a well-known prototype. Use of solid substrates with controlled morphology proves advantageous over colloidal systems for SERS applications. Moreover, such substrates are superior in terms of shelf life, packaging and ease of shipment.

Original languageEnglish
Pages (from-to)2117-2124
Number of pages8
JournalPlasmonics
Volume13
Issue number6
DOIs
Publication statusPublished - Dec 1 2018

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Muramidase
Gold
Metals
Raman Spectrum Analysis
Product Packaging
Substrates
Biotechnology
Proteins
Lasers
Health
Pulsed laser deposition
Precious metals
Raman scattering
Large scale systems
Labels
Packaging

Keywords

  • Label-free detection
  • Laser ablation
  • Lysozyme
  • Nanostructured substrates
  • Protein binding
  • Surface-enhanced Raman scattering

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biochemistry

Cite this

Protein-Metal Interactions Probed by SERS : Lysozyme on Nanostructured Gold Surface. / Agarwal, N. R.; Tommasini, M.; Ciusani, E.; Lucotti, A.; Trusso, S.; Ossi, P. M.

In: Plasmonics, Vol. 13, No. 6, 01.12.2018, p. 2117-2124.

Research output: Contribution to journalArticle

Agarwal, N. R. ; Tommasini, M. ; Ciusani, E. ; Lucotti, A. ; Trusso, S. ; Ossi, P. M. / Protein-Metal Interactions Probed by SERS : Lysozyme on Nanostructured Gold Surface. In: Plasmonics. 2018 ; Vol. 13, No. 6. pp. 2117-2124.
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