Protein modulation in mouse heart under acute and chronic hypoxia

Agnese Viganó, Michele Vasso, Anna Caretti, Valentina Bravatá, Laura Terraneo, Chiara Fania, Daniele Capitanio, Michele Samaja, Cecilia Gelfi

Research output: Contribution to journalArticle

Abstract

Exploring cellular mechanisms underlying beneficial and detrimental responses to hypoxia represents the object of the present study. Signaling molecules controlling adaptation to hypoxia (HIF-1α), energy balance (AMPK), mitochondrial biogenesis (PGC-1α), autophagic/apoptotic processes regulation and proteomic dysregulation were assessed. Responses to acute hypoxia (AH) and chronic hypoxia (CH) in mouse heart proteome were detected by 2-D DIGE, mass spectrometry and antigen-antibody reactions. Both in AH and CH, the results indicated a deregulation of proteins related to sarcomere stabilization and muscle contraction. Neither in AH nor in CH the HIF-1α stabilization was observed. In AH, the metabolic adaptation to lack of oxygen was controlled by AMPK activation and sustained by an upregulation of adenosylhomocysteinase and acetyl-CoA synthetase. AH was characterized by the mitophagic protein Bnip 3 increment. PGC-1α, a master regulator of mitochondrial biogenesis, was down-regulated. CH was characterized by the up-regulation of enzymes involved in antioxidant defense, in aldehyde bio-product detoxification and in misfolded protein degradation. In addition, a general down-regulation of enzymes controlling anaerobic metabolism was observed. After 10 days of hypoxia, cardioprotective molecules were substantially decreased whereas pro-apoptotic molecules increased accompained by downregulation of specific target proteins.

Original languageEnglish
Pages (from-to)4202-4217
Number of pages16
JournalProteomics
Volume11
Issue number21
DOIs
Publication statusPublished - 2012

Fingerprint

Modulation
AMP-Activated Protein Kinases
Molecules
Proteins
Acetate-CoA Ligase
Adenosylhomocysteinase
Stabilization
Antigen-antibody reactions
Detoxification
Deregulation
Enzymes
Proteome
Energy balance
Metabolism
Aldehydes
Mass spectrometry
Muscle
Antioxidants
Chemical activation
Organelle Biogenesis

Keywords

  • AMPK
  • Animal proteomics
  • Apoptosis
  • Autophagy
  • Heart
  • Hypoxia

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Viganó, A., Vasso, M., Caretti, A., Bravatá, V., Terraneo, L., Fania, C., ... Gelfi, C. (2012). Protein modulation in mouse heart under acute and chronic hypoxia. Proteomics, 11(21), 4202-4217. https://doi.org/10.1002/pmic.201000804

Protein modulation in mouse heart under acute and chronic hypoxia. / Viganó, Agnese; Vasso, Michele; Caretti, Anna; Bravatá, Valentina; Terraneo, Laura; Fania, Chiara; Capitanio, Daniele; Samaja, Michele; Gelfi, Cecilia.

In: Proteomics, Vol. 11, No. 21, 2012, p. 4202-4217.

Research output: Contribution to journalArticle

Viganó, A, Vasso, M, Caretti, A, Bravatá, V, Terraneo, L, Fania, C, Capitanio, D, Samaja, M & Gelfi, C 2012, 'Protein modulation in mouse heart under acute and chronic hypoxia', Proteomics, vol. 11, no. 21, pp. 4202-4217. https://doi.org/10.1002/pmic.201000804
Viganó A, Vasso M, Caretti A, Bravatá V, Terraneo L, Fania C et al. Protein modulation in mouse heart under acute and chronic hypoxia. Proteomics. 2012;11(21):4202-4217. https://doi.org/10.1002/pmic.201000804
Viganó, Agnese ; Vasso, Michele ; Caretti, Anna ; Bravatá, Valentina ; Terraneo, Laura ; Fania, Chiara ; Capitanio, Daniele ; Samaja, Michele ; Gelfi, Cecilia. / Protein modulation in mouse heart under acute and chronic hypoxia. In: Proteomics. 2012 ; Vol. 11, No. 21. pp. 4202-4217.
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