Protein phosphorylation in Escherichia coli L. form NC-7

P. Freestone, S. Grant, M. Trinei, T. Onoda, V. Norris

Research output: Contribution to journalArticle

Abstract

Wall-less L-forms of Escherichia coli constitute an interesting, and relatively underused, model system for numerous studies of bacterial physiology including the cell cycle, intracellular structure and protein phosphorylation. Total extracts of the L-form revealed a pattern of protein phosphorylation similar to that of an enteropathogenic strain but very different from its parental K-12 strain. In particular, the L-form extract revealed phosphorylation on tyrosine of a protein important in pathogenesis, TypA, and calcium-specific phosphorylation of a 40 kDa protein. Two new phosphoproteins were identified in the L-form as the DNA-binding protein Dps, and YfiD, a protein of 14 kDa with homology to pyruvate formate-lyase and a region containing a tRNA cluster in bacteriophage T5.

Original languageEnglish
Pages (from-to)3289-3295
Number of pages7
JournalMicrobiology
Volume144
Issue number12
Publication statusPublished - 1998

Fingerprint

L Forms
Phosphorylation
Escherichia coli
formate C-acetyltransferase
Proteins
Bacterial Physiological Phenomena
Phosphoproteins
DNA-Binding Proteins
Transfer RNA
Bacteriophages
Tyrosine
Cell Cycle
Calcium

Keywords

  • Calcium
  • Dps
  • TypA
  • Tyrosine phosphorylation
  • YfiD

ASJC Scopus subject areas

  • Microbiology

Cite this

Freestone, P., Grant, S., Trinei, M., Onoda, T., & Norris, V. (1998). Protein phosphorylation in Escherichia coli L. form NC-7. Microbiology, 144(12), 3289-3295.

Protein phosphorylation in Escherichia coli L. form NC-7. / Freestone, P.; Grant, S.; Trinei, M.; Onoda, T.; Norris, V.

In: Microbiology, Vol. 144, No. 12, 1998, p. 3289-3295.

Research output: Contribution to journalArticle

Freestone, P, Grant, S, Trinei, M, Onoda, T & Norris, V 1998, 'Protein phosphorylation in Escherichia coli L. form NC-7', Microbiology, vol. 144, no. 12, pp. 3289-3295.
Freestone P, Grant S, Trinei M, Onoda T, Norris V. Protein phosphorylation in Escherichia coli L. form NC-7. Microbiology. 1998;144(12):3289-3295.
Freestone, P. ; Grant, S. ; Trinei, M. ; Onoda, T. ; Norris, V. / Protein phosphorylation in Escherichia coli L. form NC-7. In: Microbiology. 1998 ; Vol. 144, No. 12. pp. 3289-3295.
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