Protein redox status in the vitreous of diabetic patients

L. Cardia, T. Micelli Ferrari, M. Vetrugno, F. Boscia, G. Durante, I. Grattagliano, G. Vendemiale, E. Altomare

Research output: Contribution to journalArticle

Abstract

PURPOSE: Little is known about the redox status of the vitreous proteins in diabetic subjects. Therefore, the aim of this study was to invastigate the content of sulfhydryl proteins (P-SH), protein mixed disulfides (PB-SH) and carbonyl proteins(DNPH) in the vitreous of diabetic and non diabetic subjects.METHODS: 27 subjects were included in the study (A=7 healthy controls, B=10 type-2 diabetic subjects with proliferative retinopathy; C=10 type-2 diabetic subjects with non-proliferative retinopathy). The vitreous was centrifuged and the supernatant analyzed for PB-SH and DNPH, indices of oxidatively demaged proteins, and P-SH, index of reduced status of the proteins.RESULTS:A significant lower content of P-SH was found in the vitreous of diabetics (9-7;±2.4 vs 17±5.4 nmol/mg prot, p 0.001), especially when a retinal complication was present(7.1±2.1 nmol/mg prot, p 0.02 compared to C).Moreover an increased PB-SH and DNPH was noticed in diabetic subjects compared to healthy controls (p 0.001). Yet, these parameters resulted to be particularly altered when diabetes was associated with retinal disease.CONCLUSIONS: This study shows an alteration of the protein redox status in subjects affected by diabetes mellitus.Vitreous proteins were found to be oxidized to a larger extend in the presence of retinal disease.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
Publication statusPublished - Feb 15 1996

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Oxidation-Reduction
Proteins
Retinal Diseases
Disulfides
Diabetes Mellitus

ASJC Scopus subject areas

  • Ophthalmology

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Cardia, L., Micelli Ferrari, T., Vetrugno, M., Boscia, F., Durante, G., Grattagliano, I., ... Altomare, E. (1996). Protein redox status in the vitreous of diabetic patients. Investigative Ophthalmology and Visual Science, 37(3).

Protein redox status in the vitreous of diabetic patients. / Cardia, L.; Micelli Ferrari, T.; Vetrugno, M.; Boscia, F.; Durante, G.; Grattagliano, I.; Vendemiale, G.; Altomare, E.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Cardia, L, Micelli Ferrari, T, Vetrugno, M, Boscia, F, Durante, G, Grattagliano, I, Vendemiale, G & Altomare, E 1996, 'Protein redox status in the vitreous of diabetic patients', Investigative Ophthalmology and Visual Science, vol. 37, no. 3.
Cardia L, Micelli Ferrari T, Vetrugno M, Boscia F, Durante G, Grattagliano I et al. Protein redox status in the vitreous of diabetic patients. Investigative Ophthalmology and Visual Science. 1996 Feb 15;37(3).
Cardia, L. ; Micelli Ferrari, T. ; Vetrugno, M. ; Boscia, F. ; Durante, G. ; Grattagliano, I. ; Vendemiale, G. ; Altomare, E. / Protein redox status in the vitreous of diabetic patients. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
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AU - Micelli Ferrari, T.

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AU - Grattagliano, I.

AU - Vendemiale, G.

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