Abstract
PURPOSE: Little is known about the redox status of the vitreous proteins in diabetic subjects. Therefore, the aim of this study was to invastigate the content of sulfhydryl proteins (P-SH), protein mixed disulfides (PB-SH) and carbonyl proteins(DNPH) in the vitreous of diabetic and non diabetic subjects.METHODS: 27 subjects were included in the study (A=7 healthy controls, B=10 type-2 diabetic subjects with proliferative retinopathy; C=10 type-2 diabetic subjects with non-proliferative retinopathy). The vitreous was centrifuged and the supernatant analyzed for PB-SH and DNPH, indices of oxidatively demaged proteins, and P-SH, index of reduced status of the proteins.RESULTS:A significant lower content of P-SH was found in the vitreous of diabetics (9-7;±2.4 vs 17±5.4 nmol/mg prot, p 0.001), especially when a retinal complication was present(7.1±2.1 nmol/mg prot, p 0.02 compared to C).Moreover an increased PB-SH and DNPH was noticed in diabetic subjects compared to healthy controls (p 0.001). Yet, these parameters resulted to be particularly altered when diabetes was associated with retinal disease.CONCLUSIONS: This study shows an alteration of the protein redox status in subjects affected by diabetes mellitus.Vitreous proteins were found to be oxidized to a larger extend in the presence of retinal disease.
| Original language | English |
|---|---|
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 37 |
| Issue number | 3 |
| Publication status | Published - Feb 15 1996 |
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ASJC Scopus subject areas
- Ophthalmology
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Protein redox status in the vitreous of diabetic patients. / Cardia, L.; Micelli Ferrari, T.; Vetrugno, M.; Boscia, F.; Durante, G.; Grattagliano, I.; Vendemiale, G.; Altomare, E.
In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protein redox status in the vitreous of diabetic patients
AU - Cardia, L.
AU - Micelli Ferrari, T.
AU - Vetrugno, M.
AU - Boscia, F.
AU - Durante, G.
AU - Grattagliano, I.
AU - Vendemiale, G.
AU - Altomare, E.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - PURPOSE: Little is known about the redox status of the vitreous proteins in diabetic subjects. Therefore, the aim of this study was to invastigate the content of sulfhydryl proteins (P-SH), protein mixed disulfides (PB-SH) and carbonyl proteins(DNPH) in the vitreous of diabetic and non diabetic subjects.METHODS: 27 subjects were included in the study (A=7 healthy controls, B=10 type-2 diabetic subjects with proliferative retinopathy; C=10 type-2 diabetic subjects with non-proliferative retinopathy). The vitreous was centrifuged and the supernatant analyzed for PB-SH and DNPH, indices of oxidatively demaged proteins, and P-SH, index of reduced status of the proteins.RESULTS:A significant lower content of P-SH was found in the vitreous of diabetics (9-7;±2.4 vs 17±5.4 nmol/mg prot, p 0.001), especially when a retinal complication was present(7.1±2.1 nmol/mg prot, p 0.02 compared to C).Moreover an increased PB-SH and DNPH was noticed in diabetic subjects compared to healthy controls (p 0.001). Yet, these parameters resulted to be particularly altered when diabetes was associated with retinal disease.CONCLUSIONS: This study shows an alteration of the protein redox status in subjects affected by diabetes mellitus.Vitreous proteins were found to be oxidized to a larger extend in the presence of retinal disease.
AB - PURPOSE: Little is known about the redox status of the vitreous proteins in diabetic subjects. Therefore, the aim of this study was to invastigate the content of sulfhydryl proteins (P-SH), protein mixed disulfides (PB-SH) and carbonyl proteins(DNPH) in the vitreous of diabetic and non diabetic subjects.METHODS: 27 subjects were included in the study (A=7 healthy controls, B=10 type-2 diabetic subjects with proliferative retinopathy; C=10 type-2 diabetic subjects with non-proliferative retinopathy). The vitreous was centrifuged and the supernatant analyzed for PB-SH and DNPH, indices of oxidatively demaged proteins, and P-SH, index of reduced status of the proteins.RESULTS:A significant lower content of P-SH was found in the vitreous of diabetics (9-7;±2.4 vs 17±5.4 nmol/mg prot, p 0.001), especially when a retinal complication was present(7.1±2.1 nmol/mg prot, p 0.02 compared to C).Moreover an increased PB-SH and DNPH was noticed in diabetic subjects compared to healthy controls (p 0.001). Yet, these parameters resulted to be particularly altered when diabetes was associated with retinal disease.CONCLUSIONS: This study shows an alteration of the protein redox status in subjects affected by diabetes mellitus.Vitreous proteins were found to be oxidized to a larger extend in the presence of retinal disease.
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UR - http://www.scopus.com/inward/citedby.url?scp=33750195896&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33750195896
VL - 37
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 0146-0404
IS - 3
ER -