Proteins and protein fragments in nephrotic syndrome: Clusters, specificity and mechanisms

Giovanni Candiano, Maurizio Bruschi, Andrea Petrotto, Laura Santucci, Piero Del Boccio, Andrea Urbani, Elisabetta Bertoni, Rosanna Gusmano, Maurizio Salvadori, Francesco Scolari, Gian Marco Ghiggeri

Research output: Contribution to journalArticle

Abstract

Proteinuria is the hallmark of renal diseases and the characterization of the urinary protein composition may become an important source of information for diagnosis and research. So far, protein analysis in urine has been utilized for a generic individuation of site-specific defects (glomerular vs. tubular) but there is a need for an extension of proteomics to specific urinary biomarkers in selected clinical conditions. The identification of fragments of proteins in plasma and urine may increase the spectrum of urinary biomarkers. The unique speculative application so far proposed for protein fragments is nephrotic syndrome, and specifically focal segmental glomerulosclerosis, in which case they reflect intrinsic proteolysis occurring in plasma and represent surrogate biomarkers of the disease activity. Albumin is probably the most studied protein. Several of the albumin fragments present a peculiar distribution of the fingerprint peptide pattern containing both the N-terminal region and the C-terminal domain with a complete lack of any MS signals for the internal sequence region. Their characterization utilizing new strategies based on 2-D nondenaturing electrophoresis is now in progress. Studies on a direct characterization of proteases in plasma and urine will also define the participation of proteases to the genesis of renal diseases.

Original languageEnglish
Pages (from-to)956-963
Number of pages8
JournalProteomics - Clinical Applications
Volume2
Issue number7-8
DOIs
Publication statusPublished - Jul 2008

Keywords

  • Albumin fragments
  • Nephrotic syndrome
  • Urinary peptides
  • Urinary proteins

ASJC Scopus subject areas

  • Clinical Biochemistry

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    Candiano, G., Bruschi, M., Petrotto, A., Santucci, L., Del Boccio, P., Urbani, A., Bertoni, E., Gusmano, R., Salvadori, M., Scolari, F., & Ghiggeri, G. M. (2008). Proteins and protein fragments in nephrotic syndrome: Clusters, specificity and mechanisms. Proteomics - Clinical Applications, 2(7-8), 956-963. https://doi.org/10.1002/prca.200780157