Proteins differentially expressed in human beta-cells-enriched pancreatic islet cultures and human insulinomas

Letícia F. Terra, Priscila C. Teixeira, Rosangela A M Wailemann, André Zelanis, Giuseppe Palmisano, Edecio Cunha-Neto, Jorge Kalil, Martin R. Larsen, Leticia Labriola, Mari C. Sogayar

Research output: Contribution to journalArticle

Abstract

In view of the great demand for human beta-cells for physiological and medical studies, we generated cell lines derived from human insulinomas which secrete insulin, C-peptide and express neuroendocrine and islet markers. In this study, we set out to characterize their proteomes, comparing them to those of primary beta-cells using DIGE followed by MS. The results were validated by Western blotting. An average of 1800 spots was detected with less than 1% exhibiting differential abundance. Proteins more abundant in human islets, such as Caldesmon, are involved in the regulation of cell contractility, adhesion dependent signaling, and cytoskeletal organization. In contrast, almost all proteins more abundant in insulinoma cells, such as MAGE2, were first described here and could be related to cell survival and resistance to chemotherapy. Our proteomic data provides, for the first time, a molecular snapshot of the orchestrated changes in expression of proteins involved in key processes which could be correlated with the altered phenotype of human beta-cells. Collectively our observations prompt research towards the establishment of bioengineered human beta-cells providing a new and needed source of cultured human beta-cells for beta-cell research, along with the development of new therapeutic strategies for detection, characterization and treatment of insulinomas.

Original languageEnglish
Pages (from-to)16-25
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume381
Issue number1-2
DOIs
Publication statusPublished - Dec 5 2013

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Keywords

  • Diabetes
  • Insulinomas
  • Islets
  • Mammalian biology
  • Mass spectrometry
  • Pancreatic cell biology

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

Terra, L. F., Teixeira, P. C., Wailemann, R. A. M., Zelanis, A., Palmisano, G., Cunha-Neto, E., Kalil, J., Larsen, M. R., Labriola, L., & Sogayar, M. C. (2013). Proteins differentially expressed in human beta-cells-enriched pancreatic islet cultures and human insulinomas. Molecular and Cellular Endocrinology, 381(1-2), 16-25. https://doi.org/10.1016/j.mce.2013.07.004